Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Virgibacillus salexigens |
Protein Variants | Comment | Organism |
---|---|---|
A163C | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
A163C/S244C | the mutant shows increased activity compared to the wild type enzyme | Virgibacillus salexigens |
D148A | inactive | Virgibacillus salexigens |
D148E | inactive | Virgibacillus salexigens |
F143A | inactive | Virgibacillus salexigens |
F143W | inactive | Virgibacillus salexigens |
F143Y | inactive | Virgibacillus salexigens |
F242A | inactive | Virgibacillus salexigens |
F242W | inactive | Virgibacillus salexigens |
F242Y | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
F263A | inactive | Virgibacillus salexigens |
F263W | inactive | Virgibacillus salexigens |
F263Y | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
F95A | inactive | Virgibacillus salexigens |
H146A | inactive | Virgibacillus salexigens |
H248A | inactive | Virgibacillus salexigens |
N133A | inactive | Virgibacillus salexigens |
N261A | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
P198A | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
Q129A | inactive | Virgibacillus salexigens |
R131A | inactive | Virgibacillus salexigens |
R259A | inactive | Virgibacillus salexigens |
R259H | inactive | Virgibacillus salexigens |
R259K | inactive | Virgibacillus salexigens |
R259Q | inactive | Virgibacillus salexigens |
S165A | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
S167A | the mutant shows about wild type activity | Virgibacillus salexigens |
S205A | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
S244C | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
S250A | inactive | Virgibacillus salexigens |
V265A | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
V265L | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
V265T | the mutant shows reduced activity compared to the wild type enzyme | Virgibacillus salexigens |
W152A | inactive | Virgibacillus salexigens |
W152F | inactive | Virgibacillus salexigens |
W152Y | inactive | Virgibacillus salexigens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
(4S)-ectoine | mutant A163C/S244C, at pH 7.5 and 32°C | Virgibacillus salexigens | |
5.5 | - |
(4S)-ectoine | mutant N261A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
5.9 | - |
(4S)-ectoine | wild type enzyme, at pH 7.5 and 32°C | Virgibacillus salexigens | |
6 | - |
(4S)-ectoine | mutant A163C, at pH 7.5 and 32°C | Virgibacillus salexigens | |
6.2 | - |
(4S)-ectoine | mutant S244C, at pH 7.5 and 32°C | Virgibacillus salexigens | |
6.8 | - |
(4S)-ectoine | mutant S205A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
6.9 | - |
(4S)-ectoine | mutant S167A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
7.1 | - |
(4S)-ectoine | mutant P198A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
8 | - |
(4S)-ectoine | mutant F263Y, at pH 7.5 and 32°C | Virgibacillus salexigens | |
9.7 | - |
(4S)-ectoine | mutant V265T, at pH 7.5 and 32°C | Virgibacillus salexigens | |
16.2 | - |
(4S)-ectoine | mutant V265A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
17.3 | - |
(4S)-ectoine | mutant S165A, at pH 7.5 and 32°C | Virgibacillus salexigens | |
19.6 | - |
(4S)-ectoine | mutant F242Y, at pH 7.5 and 32°C | Virgibacillus salexigens | |
24.6 | - |
(4S)-ectoine | mutant V265L, at pH 7.5 and 32°C | Virgibacillus salexigens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Virgibacillus salexigens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S)-ectoine + 2-oxoglutarate + O2 | Virgibacillus salexigens | - |
(4S,5S)-5-hydroxyectoine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Virgibacillus salexigens | Q2TDY4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S)-ectoine + 2-oxoglutarate + O2 | - |
Virgibacillus salexigens | (4S,5S)-5-hydroxyectoine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ectD | - |
Virgibacillus salexigens |