Literature summary for 1.13.11.57 extracted from

Uchendu, S.; Rafalowski, A.; Schick, D.; Cohn, E.; Hassan, B.; Taylor, E.
Identifying metabolic pathway intermediates that modulate the gallate dioxygenase (DesB) from Sphingobium sp. strain SYK-6 (2021), Process Biochem., 102, 408-416 .

No PubMed abstract available

Search for more literature for 1.13.11.57

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Sphingobium sp. SYK-6

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Methoxy-4-methylphenol	20.8% inhibition at 5 mM	Sphingobium sp. SYK-6
4-nitrocatechol	31.6% inhibition at 5 mM	Sphingobium sp. SYK-6
protocatechuate	most potent inhibitor with mixed (competitive and non-competitive) inhibition profile, 49% inhibition at 5 mM	Sphingobium sp. SYK-6
syringaldehyde	least potent inhibitor, 8.66% inhibition at 5 mM	Sphingobium sp. SYK-6
syringate	16.3% inhibition at 5 mM	Sphingobium sp. SYK-6
vanilliate	24.2% inhibition at 5 mM	Sphingobium sp. SYK-6
Vanillin	11.3% inhibition at 5 mM	Sphingobium sp. SYK-6

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.123	-	gallate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

Organism

Organism	UniProt	Comment	Textmining
Sphingobium sp. SYK-6	G2IKE5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sephadex G-25 gel filtration	Sphingobium sp. SYK-6

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gallate + O2	-	Sphingobium sp. SYK-6	(2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate	-	?

Subunits

Subunits	Comment	Organism
?	x * 49220, calculated from amino acid sequence	Sphingobium sp. SYK-6

Synonyms

Synonyms	Comment	Organism
DesB	-	Sphingobium sp. SYK-6
gallate 3,4-dioxygenase	-	Sphingobium sp. SYK-6

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
316	-	gallate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
5.48	-	protocatechuate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6
8.13	-	vanilliate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6
13.3	-	4-nitrocatechol	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

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Release 2023.1 (January 2023)