Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
R403L | a loss of electrostatic interaction between Arg403 and negatively charged amino acid residues of alpha2-helix has only minor impact on protein folding, but partially destabilizes the tertiary structure of the enzyme. Arg403Leu exchange induces strong substrate inhibition. kcat/Km values strongly decreased for linoleic acid and methyl arachidonate but almost unchanged for arachidonic acid compared to wild-type | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
arachidonic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
0.0109 | - |
arachidonic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
0.0114 | - |
methyl arachidonate | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
0.0169 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
0.018 | - |
methyl arachidonate | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
0.0281 | - |
linoleic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
linoleic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
methyl arachidonate + O2 | - |
Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12/15-lipoxygenase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryctolagus cuniculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
methyl arachidonate | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
760 | - |
methyl arachidonate | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
830 | - |
linoleic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
1410 | - |
arachidonic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
1950 | - |
arachidonic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
2650 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus |