Cloned (Comment) | Organism |
---|---|
recombinant expression of Strep-tagged enzyme | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C93G | site-directed mutagenesis | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow kinetics of wild-type and mutant enzymes | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity, an iron-oxygen intermediate is formed during the catalytic cycle of cysteine dioxygenase, Moessbauer spectroscopy analysis resuting in three different species: CDO:FeII, CDO:FeII:cysteine site I, and CDO:FeII:cysteine site II, dissociation constants, absorptions spectra, and structure analysis, detailed overview | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | Rattus norvegicus | - |
3-sulfinoalanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P21816 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | residues C93-Y157 crosslink as a result of a posttranslational modification | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged enzyme | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-cysteine + O2 = 3-sulfinoalanine | proposed catalytic cycle of CDO enzymes: the pentacoordinated resting state structure can convert to a hexacoordinated structure by binding of water. Upon binding of molecular oxygen the water molecule is displaced and an iron(III)-superoxo structure is formed. The terminal oxygen atom of the iron(III)-superoxo group attacks the sulfur of the cysteinate group to form a ring-structure, which can homogeneously split into a sulfoxide and iron(IV)-oxo species. The sulfoxide-bound complex rotates from sulfur-bound to oxygen-bound to the iron center. The latter transfers the oxygen atom from the iron(IV)-oxo group to substrate in a final step to form cysteine sulfinic acid product complex | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfinoalanine | - |
? | |
L-cysteine + O2 | anaerobic CDO reaction conditions | Rattus norvegicus | 3-sulfinoalanine | - |
? | |
additional information | formation of an active CDO:cysteine substrate complex | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |