Cloned (Comment) | Organism |
---|---|
recombinant expression of the thioredoxin-His6-tagged enzyme | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme complexed with cysteine persulfide or 3-mercaptopropionic acid persulfide, hanging drop vapor diffusion method, mixing of 0.0015 ml of 8 mg/ml protein with 0.0015 ml of reservoir solution containing 24-34% w/v PEG 4000, 100-250 mM ammonium acetate, 100 mM sodium citrate, pH 5.6, 0-4 mM dithionite, and 40 mM ligand, final pH is 6.1-6.2, 24°C, one week, X-ray diffraction structure determination and analysis at 1.63-2.05 A resolution | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P21816 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant thioredoxin-His6-tagged enzyme by affinity chromatography | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | persulfenate and persulfide binding in the active site of cysteine dioxygenase, overview | Rattus norvegicus |