Cloned (Comment) | Organism |
---|---|
when the wild-type and mutant (M129V/E293G) genes are overexpressed in Escherichia coli, unmodified or six-His-tagged proteins of the expected size are overproduced as inactive proteins. To overcome this problem, the Mn2+-dependent catalase genes are overexpressed directly in Thermus thermophilus under the control of the Pnar promoter. This promoter belongs to a respiratory nitrate reductase from of Thermus thermophilus HB8, whose transcription is activated by the combined action of nitrate and anoxia. Upon induction in Thermus thermophilus HB8, a 20fold to 30fold increase in catalase specific activity is observed, whereas a 90fold to 110fold increase is detected when the laboratory strain Thermus thermophilus HB27::nar | Thermus thermophilus |
Protein Variants | Comment | Organism |
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M129V/E293G | less thermostable mutant enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
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Thermus thermophilus | Q84DB4 | - |
- |