Cloned (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
W233F | complete loss of cytochrome c-dependent activity | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
cytochrome c | pH 7.4, 25°C | Trypanosoma cruzi | |
0.19 | - |
L-ascorbate | pH 7.4, 25°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | enzyme is closely associated with mitochondrial membranes | Trypanosoma cruzi | 5739 | - |
plasma membrane | enzyme is associated with plasma membrane | Trypanosoma cruzi | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q8I1N3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | - |
Trypanosoma cruzi | 2 monodehydroascorbate + 2 H2O | - |
? | |
cytochrome c + H2O2 | - |
Trypanosoma cruzi | ? + H2O | - |
? | |
additional information | reaction includes formation of a compound I-like product, characteristic of the generation of a tryptophanyl radical-cation at residue W233. In addition, formation of a C222-derived radical is observed. electron transfer between Trp233 and Cys222 is possible and likely to participate in the catalytic cycle | Trypanosoma cruzi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APX | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpressing strains are significantly more infective to macrophages and cardiomyocytes, as well as in the mouse model of Chagas disease than wild-type | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
L-ascorbate | pH 7.4, 25°C | Trypanosoma cruzi | |
210 | - |
cytochrome c | pH 7.4, 25°C | Trypanosoma cruzi |