Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | complete inhibition at 0.05 mM | Brassica rapa | |
NaN3 | complete inhibition at 1 mM | Brassica rapa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
H2O2 | - |
Brassica rapa | |
0.402 | - |
ascorbate | - |
Brassica rapa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | - |
Brassica rapa | 9570 | - |
cytosol | - |
Brassica rapa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Brassica rapa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | Brassica rapa | - |
dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on DEAE-Sephacel, ammonium sulfate treatment and column chromatography on butyl-Toyopearl and TSK G3000SW | Brassica rapa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica rapa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56 | - |
purified enzyme | Brassica rapa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytochrome c + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
glutathione + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
guaiacol + H2O2 | no reaction | Brassica rapa | ? | - |
? | |
L-ascorbate + H2O2 | L-ascorbate is the most effective natural electron donor | Brassica rapa | dehydroascorbate + H2O | - |
? | |
L-ascorbate + H2O2 | - |
Brassica rapa | dehydroascorbate + 2 H2O | - |
? | |
additional information | the cytosolic enzyme exhibits no activity with: glutathione, cytochrome c and NAD(P)H | Brassica rapa | ? | - |
? | |
NADPH + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
pyrogallol + H2O2 | 2.5-fold higher rate than that of L-ascorbate | Brassica rapa | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Brassica rapa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Brassica rapa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
complete loss of activity | Brassica rapa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
assay at | Brassica rapa |
6.5 | - |
- |
Brassica rapa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Brassica rapa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Brassica rapa |