KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
1-butanol | isoform ADH IIB, pH 8.0 | Pseudomonas putida | |
0.15 | - |
1-butanol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
0.226 | - |
1,2-propanediol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
2.4 | - |
glycerol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
3 | 3.2 | ethanol | isoform ADH IIG, pH 8.0 | Pseudomonas putida | |
5.61 | - |
ethanol | isoform ADH IIB, pH 8.0 | Pseudomonas putida | |
10.2 | - |
1,2-propanediol | isoform ADH IIB, pH 8.0 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
72000 | - |
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
isoform ADH IIG, pH 9.0 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + ferricyanide | ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol | Pseudomonas putida | 2-hydroxybutanal + ferrocyanide | - |
? | |
1,2-ethanediol + ferricyanide | ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol | Pseudomonas putida | hydroxyacetaldehyde + ferrocyanide | - |
? | |
1,2-propanediol + ferricyanide | ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity | Pseudomonas putida | 2-hydroxypropanal + ferrocyanide | - |
? | |
1,3-butanediol + ferricyanide | ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxybutanal + ferrocyanide | - |
? | |
1,3-propandione + ferricyanide | ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxypropanal + ferrocyanide | - |
? | |
1,4-butanediol + ferricyanide | ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol | Pseudomonas putida | 4-hydroxybutanal + ferrocyanide | - |
? | |
1-butanol + ferricyanide | ADH IIG, 42% of the activity with 1,2-propanediol | Pseudomonas putida | butanal + ferrocyanide | - |
? | |
1-heptanol + ferricyanide | ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol | Pseudomonas putida | heptanal + ferrocyanide | - |
? | |
1-hexanol + ferricyanide | ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol | Pseudomonas putida | hexanal + ferrocyanide | - |
? | |
1-octanol + ferricyanide | ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol | Pseudomonas putida | octanal + ferrocyanide | - |
? | |
1-pentanol + ferricyanide | ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol | Pseudomonas putida | pentanal + ferrocyanide | - |
? | |
1-propanol + ferricyanide | ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol | Pseudomonas putida | propanal + ferrocyanide | - |
? | |
2,3-butanediol + ferricyanide | ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol | Pseudomonas putida | 3-hydroxybutan-2-one + ferrocyanide | - |
? | |
2,4-pentanediol + ferricyanide | ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol | Pseudomonas putida | 4-hydroxypentan-2-one + ferrocyanide | - |
? | |
2-butanol + ferricyanide | ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol | Pseudomonas putida | butanone + ferrocyanide | - |
? | |
2-hexanol + ferricyanide | ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol | Pseudomonas putida | hexan-2-one + ferrocyanide | - |
? | |
2-pentanol + ferricyanide | ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol | Pseudomonas putida | pentan-2-one + ferrocyanide | - |
? | |
2-propanol + ferricyanide | ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol | Pseudomonas putida | acetone + ferrocyanide | - |
? | |
3-pentanol + ferricyanide | ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol | Pseudomonas putida | pentan-3-one + ferrocyanide | - |
? | |
allyl alcohol + ferricyanide | ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol | Pseudomonas putida | prop-2-enal + ferrocyanide | - |
? | |
butanal + ferrocyanide | ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol | Pseudomonas putida | 1-butanol + ferricyanide | - |
? | |
ethanal + ferrocyanide | ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol | Pseudomonas putida | ethanol + ferricyanide | - |
? | |
ethanol + ferricyanide | ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol | Pseudomonas putida | ethanal + ferrocyanide | - |
? | |
glycerol + ferrocyanide | ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol | Pseudomonas putida | 2,3-dihydroxypropanal + ferricyanide | - |
? | |
iso-amyl alcohol + ferricyanide | ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol | Pseudomonas putida | 3-methylbutanol + ferrocyanide | - |
? | |
propanal + ferrocyanide | ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol | Pseudomonas putida | 1-propanol + ferricyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ADH IIB | - |
Pseudomonas putida |
ADH IIG | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | both isoforms ADH IIB and ADH IIG | Pseudomonas putida | |
pyrroloquinoline quinone | both isoforms ADH IIB and ADH IIG | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | ADH IIB is formed in the cells grown on alcohols of medium chain length and also on 1,3-butanediol. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol, of which the former alcohol is more effective | Pseudomonas putida |