Any feedback?
Literature summary for 1.1.3.B4 extracted from
- Glycerol oxidase, a novel copper hemoprotein from Aspergillus japonicus. Molecular and catalytic properties of the enzyme and its application to the analysis of serum triglycerides (1984), J. Biol. Chem., 259, 2748-2753.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Borate | exposure of the enzyme to borate buffer of pH 10.0 results in activation of the enzyme | Aspergillus japonicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 25% inhibition at 0.01 mM | Aspergillus japonicus | |
| 8-hydroxyquinoline | 7.9% inhibition at 1 mM | Aspergillus japonicus | |
| diethyldithiocarbamate | 40.1% inhibition at 1 mM | Aspergillus japonicus | |
| dimercapto-1-propanol | 56.3% inhibition at 0.01 mM | Aspergillus japonicus | |
| dithiothreitol | 26.5% inhibition at 0.01 mM | Aspergillus japonicus | |
| ethyl xanthate | 27.1% inhibition at 0.1 mM | Aspergillus japonicus | |
| L-ascorbic acid | 31.2% inhibition at 0.1 mM | Aspergillus japonicus | |
| L-cysteine | 12.2% inhibition at 0.1 mM | Aspergillus japonicus | |
| reduced glutathione | 40.8% inhibition at 0.1 mM | Aspergillus japonicus | |
| Salicylaldoxime | 11.2% inhibition at 1 mM | Aspergillus japonicus | |
| sodium sulfite | 46.2% inhibition at 0.1 mM | Aspergillus japonicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | contains 2 atoms of copper per mol of enzyme | Aspergillus japonicus | |
| Mg2+ | activates | Aspergillus japonicus | |
| Mn2+ | activates | Aspergillus japonicus | |
| additional information | the enzyme does not contain molybdenum and manganese | Aspergillus japonicus | |
| Ni2+ | activates | Aspergillus japonicus | |
| Zn2+ | contains 0.76 atoms of zinc per mol of enzyme. Zn2+ is the most effective cation with activation being 2.3fold at a concentration of 1.0 mM | Aspergillus japonicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 400000 | - |
sedimentation equilibrium method | Aspergillus japonicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + O2 | Aspergillus japonicus | - |
glyceraldehyde + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus japonicus | - |
- |
- |
| Aspergillus japonicus AT 008 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephadex A-25 column chromatography, hydroxylapatite column chromatography, and Sephadex G-200 gel filtration | Aspergillus japonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, activated enzyme at pH 8.0-10.0, 1 week, no loss of activity | Aspergillus japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-butanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
| 1,3-butanediol + O2 | - |
Aspergillus japonicus AT 008 | ? | - |
? | |
| 1,3-propanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
| 1,4-butanediol + O2 | - |
Aspergillus japonicus | ? | - |
? | |
| 1,4-butanediol + O2 | - |
Aspergillus japonicus AT 008 | ? | - |
? | |
| D-fructose + O2 | slight oxidation activity | Aspergillus japonicus | ? | - |
? | |
| D-fructose + O2 | slight oxidation activity | Aspergillus japonicus AT 008 | ? | - |
? | |
| D-galactose + O2 | slight oxidation activity | Aspergillus japonicus | ? | - |
? | |
| D-galactose + O2 | slight oxidation activity | Aspergillus japonicus AT 008 | ? | - |
? | |
| dihydroxyacetone + O2 | 58.8% activity compared to glycerol | Aspergillus japonicus | 3-hydroxy-2-oxopropanal + H2O2 | - |
? | |
| glycerol + O2 | - |
Aspergillus japonicus | glyceraldehyde + H2O2 | - |
? | |
| additional information | no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol | Aspergillus japonicus | ? | - |
? | |
| additional information | no activity with glycerol 3-phosphate, methanol, ethanol, and polyvinyl alcohol | Aspergillus japonicus AT 008 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | the enzyme is stable up to 45°C and loses about 40% of activity at 50°C in 50 mM TES buffer, pH 7.0 | Aspergillus japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus japonicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| protoheme IX | the enzyme contains 0.94 mol of heme per mol of enzyme | Aspergillus japonicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
dimercapto-1-propanol | at pH 7.0 and 37°C | Aspergillus japonicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
