KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
L-gulono-1,4-lactone | - |
Rattus norvegicus | |
0.15 | - |
L-gulono-1,4-lactone | - |
Capra hircus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, SDS-PAGE | Rattus norvegicus |
51000 | - |
x * 51000, SDS-PAGE | Capra hircus |
500000 | - |
gel filtration | Rattus norvegicus |
500000 | - |
gel filtration | Capra hircus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gulono-1,4-lactone + O2 | Rattus norvegicus | - |
L-xylo-hex-3-ulonolactone + H2O2 | - |
? | |
L-gulono-1,4-lactone + O2 | Capra hircus | - |
L-xylo-hex-3-ulonolactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capra hircus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Capra hircus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 | product isomerizes spontaneously to L-ascorbate | Rattus norvegicus | |
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 | product isomerizes spontaneously to L-ascorbate | Capra hircus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
- |
Rattus norvegicus |
2.05 | - |
- |
Capra hircus |
Storage Stability | Organism |
---|---|
-20 C, 1 month, 100% activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gulono-1,4-lactone + O2 | - |
Rattus norvegicus | L-xylo-hex-3-ulonolactone + H2O2 | - |
? | |
L-gulono-1,4-lactone + O2 | - |
Capra hircus | L-xylo-hex-3-ulonolactone + H2O2 | - |
? | |
L-gulono-1,4-lactone + O2 | - |
Rattus norvegicus | L-xylo-hex-3-ulonolactone + H2O2 | product isomerizes spontaneously to L-ascorbate | ? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 51000, SDS-PAGE | Rattus norvegicus |
oligomer | x * 51000, SDS-PAGE | Capra hircus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rattus norvegicus |
37 | - |
- |
Capra hircus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
7.5 | - |
- |
Capra hircus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | covalently bound, flavoenzyme | Rattus norvegicus | |
flavin | covalently bound, flavoenzyme | Capra hircus |