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Literature summary for 1.1.3.8 extracted from

  • Krasnov, A.; Reinisalo, M.; Pitk§nen, T.I.; Nishikimi, M.; M¸lsa, H.
    Expression of rat gene L-gulono-gamma-lactone oxidase, the key enzyme of L-ascorbic acid biosynthesis, in guinea pig cells and in teleost fish rainbow trout (Oncorhynchus mykiss) (1998), Biochim. Biophys. Acta, 1381, 241-248.
    View publication on PubMed
Search for more literature for 1.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109, transfected into guinea pig cell line 104C1 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome integral membrane protein in the endoplasmic reticulum Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulono-1,4-lactone + O2 Rattus norvegicus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Cavia porcellus
-
 guinea pig
-
no activity in Oncorhynchus mykiss
-
 teleost fish rainbow trout
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 after transfection in guinea pig cells Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?

Synonyms

Synonyms Comment Organism
GLO
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
flavin covalently bound, flavoenzyme Rattus norvegicus