Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, recombinant expression in Pichia pastoris | Ustilago maydis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Hydroxybenzyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis | |
anisyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis | |
cinnamyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Ustilago maydis | |
0.0048 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
0.015 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
0.035 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
0.042 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
0.12 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
1.82 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Ustilago maydis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ustilago maydis | - |
strains BRFM 1093 and 521/FGSC 9021 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme contains no putative N/O-glycosylation sites | Ustilago maydis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Ustilago maydis grown on maize | Ustilago maydis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dimethoxybenzyl alcohol + O2 | - |
Ustilago maydis | 2,4-dimethoxybenzaldehyde + H2O2 | - |
? | |
2,4-hexadien-1-ol + O2 | - |
Ustilago maydis | ? + H2O2 | - |
? | |
4-hydroxybenzyl alcohol + O2 | - |
Ustilago maydis | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | - |
Ustilago maydis | 4-methoxybenzaldehyde + H2O2 | - |
? | |
anisyl alcohol + O2 | best substrate | Ustilago maydis | anisaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Ustilago maydis | benzaldehyde + H2O2 | - |
? | |
cinnamyl alcohol + O2 | - |
Ustilago maydis | cinnamaldehyde + H2O2 | - |
? | |
additional information | the enzyme is able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate is obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. Assay method using ABTS [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)]/horseradish peroxidase. The enzyme shows no activity as a GMC oxidoreductase | Ustilago maydis | ? | - |
? | |
veratryl alcohol + O2 | i.e. 3,4-dimethoxybenzyl alcohol | Ustilago maydis | veratrylaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme homology structure modeling | Ustilago maydis |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Ustilago maydis |
um04044 | - |
Ustilago maydis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ustilago maydis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
45 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
53 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
64 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
75 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
88 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Ustilago maydis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Ustilago maydis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the AA3_2 subfamily, phylogenetic analysis | Ustilago maydis |
additional information | enzyme homology structure modeling | Ustilago maydis |
physiological function | the aryl-alcohol oxidase acts on the lignin fraction in biomass | Ustilago maydis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.5 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
440 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
1790 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
2510 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
4270 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
9380 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis |