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Literature summary for 1.1.3.7 extracted from

  • Ferreira, P.; Hernandez-Ortega, A.; Herguedas, B.; Rencoret, J.; Gutierrez, A.; Martinez, M.J.; Jimenez-Barbero, J.; Medina, M.; Martinez, A.T.
    Kinetic and chemical characterization of aldehyde oxidation by fungal aryl-alcohol oxidase (2010), Biochem. J., 425, 585-593.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
H502S the mutant shows much lower activities on 4-nitrobenzaldehyde (340fold activity decrease) than the wild type enzyme Pleurotus eryngii
H546S the mutant shows much lower activities on 4-nitrobenzaldehyde (670fold activity decrease) than the wild type enzyme Pleurotus eryngii
Y92F the mutation causes a 5fold reduction in the p-anisaldehyde kcat value Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.7
-
3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.8
-
4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.5
-
3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
2
-
4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
2.2
-
3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
3
-
3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
4.7
-
4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
4.9
-
4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
5
-
4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
7
-
benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
8
-
veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
13
-
2,4-hexadienal wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94219
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-hexadien-1-ol + O2
-
Pleurotus eryngii 2,4-hexadienal + H2O2
-
?
2,4-hexadienal + O2
-
Pleurotus eryngii 2,4-hexadienoate + H2O2
-
?
3,4-difluorobenzaldehyde + O2
-
Pleurotus eryngii 3,4-difluorobenzoic acid + H2O2
-
?
3,4-difluorobenzaldehyde + O2
-
Pleurotus eryngii 3,4-difluorobenzoic acid + H2O2
-
r
3-chloro-4-anisaldehyde + O2
-
Pleurotus eryngii 3-chloro-4-anisic acid + H2O2
-
r
3-chloro-4-anisyl alcohol + O2
-
Pleurotus eryngii 3-chloro-4-anisaldehyde + H2O2
-
?
3-chlorobenzaldehyde + O2
-
Pleurotus eryngii 3-chlorobenzoic acid + H2O2
-
r
3-chlorobenzyl alcohol + O2
-
Pleurotus eryngii 3-chlorobenzaldehyde + H2O2
-
?
3-fluorobenzaldehyde + O2
-
Pleurotus eryngii 3-fluorobenzoic acid + H2O2
-
r
3-fluorobenzyl alcohol + O2
-
Pleurotus eryngii 3-fluorobenzaldehyde + H2O2
-
?
4-anisaldehyde + O2
-
Pleurotus eryngii 4-anisic acid + H2O2
-
r
4-anisyl alcohol + O2
-
Pleurotus eryngii 4-anisaldehyde + H2O2
-
?
4-chlorobenzaldehyde + O2
-
Pleurotus eryngii 4-chlorobenzoic acid + H2O2
-
r
4-chlorobenzyl alcohol + O2
-
Pleurotus eryngii 4-chlorobenzaldehyde + H2O2
-
?
4-fluorobenzaldehyde + O2
-
Pleurotus eryngii 4-fluorobenzoic acid + H2O2
-
r
4-fluorobenzyl alcohol + O2
-
Pleurotus eryngii 4-fluorobenzaldehyde + H2O2
-
?
4-nitrobenzaldehyde + O2
-
Pleurotus eryngii 4-nitrobenzoic acid + H2O2
-
?
4-nitrobenzaldehyde + O2
-
Pleurotus eryngii 4-nitrobenzoic acid + H2O2
-
r
benzaldehyde + O2
-
Pleurotus eryngii benzoic acid + H2O2
-
r
benzyl alcohol + O2
-
Pleurotus eryngii benzaldehyde + H2O2
-
?
additional information AAO typically oxidizes aromatic alcohols to the corresponding aldehydes. However, the enzyme can also oxidize aromatic aldehydes to the corresponding acids Pleurotus eryngii ?
-
?
veratraldehyde + O2
-
Pleurotus eryngii veratric acid + H2O2
-
r
veratryl alcohol + O2
-
Pleurotus eryngii veratraldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
AAO
-
Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.05
-
4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.057
-
3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.13
-
veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.33
-
2,4-hexadienal wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.367
-
4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.5
-
benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.85
-
3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.867
-
3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.883
-
3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.05
-
4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.21
-
4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.633
-
4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pleurotus eryngii

General Information

General Information Comment Organism
physiological function aryl-alcohol oxidase provides H2O2 for lignin biodegradation Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.013
-
4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.0167
-
veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.025
-
2,4-hexadienal wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.073
-
benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.075
-
4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.085
-
3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.087
-
4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.223
-
4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.282
-
3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.315
-
4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.407
-
3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.597
-
4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
0.643
-
3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii