KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
veratryl alcohol | pH 6.0, 40°C | Pleurotus ostreatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
three-phase partitioning with 30% (w/v) of ammonium sulfate and t-butanol (1:1 ratio) | Pleurotus ostreatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.155 | - |
crude enzyme, using veratryl alcohol as substrate, at pH 6.0, 40°C | Pleurotus ostreatus |
1.58 | - |
after 10.19fold purification, using veratryl alcohol as substrate, at pH 6.0, 40°C | Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-anisyl alcohol + O2 | best substrate | Pleurotus ostreatus | 4-anisaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Pleurotus ostreatus | benzaldehyde + H2O2 | - |
? | |
veratryl alcohol + O2 | - |
Pleurotus ostreatus | veratraldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Pleurotus ostreatus |
aryl alcohol oxidase | - |
Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pleurotus ostreatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme completely loses its activity at 60°C | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Pleurotus ostreatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | the enzyme displays over 63% of its activity in the pH range of 4.0-7.0. A further increase of these pH values decreases the relative activity to 17% | Pleurotus ostreatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pleurotus ostreatus |
Organism | Comment | Expression |
---|---|---|
Pleurotus ostreatus | compared with the control, inducers increased the AAO production in Pleurotus ostreatus in the following order: L-tyrosine (5 mM) > veratryl alcohol (7 mM) > benzyl alcohol (10 mM) > L-phenylalanine (5 mM) > 4-anisyl alcohol (9 mM) | up |
General Information | Comment | Organism |
---|---|---|
physiological function | aryl alcohol oxidase is involved in lignin degradation | Pleurotus ostreatus |