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Literature summary for 1.1.3.7 extracted from

  • Ruiz-Duenas, F.J.; Ferreira, P.; Martinez, M.J.; Martinez, A.T.
    In vitro activation, purification, and characterization of Escherichia coli expressed aryl-alcohol oxidase, a unique H2O2-producing enzyme (2006), Protein Expr. Purif., 45, 191-199.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene aao, expression of FLAG1-tagged enzyme in Escherichia coli strain W3110, the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies, expression iand glycosylation of the enzyme in Emericella nidulans strain IJFM A729 Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
4-anisyl alcohol pH 8.0, 25°C, recombinant enzyme from Emericella nidulans Pleurotus eryngii
0.037
-
4-anisyl alcohol pH 8.0, 25°C, recombinant enzyme from Escherichia coli Pleurotus eryngii
0.04
-
4-anisyl alcohol pH 8.0, 25°C, wild-type enzyme Pleurotus eryngii
0.092
-
2,4-hexadien-1-ol pH 8.0, 25°C, recombinant enzyme from Emericella nidulans Pleurotus eryngii
0.12
-
2,4-hexadien-1-ol pH 8.0, 25°C, recombinant enzyme from Escherichia coli Pleurotus eryngii
0.22
-
3-anisyl alcohol pH 8.0, 25°C, wild-type enzyme Pleurotus eryngii
0.269
-
3-anisyl alcohol pH 8.0, 25°C, recombinant enzyme from Escherichia coli Pleurotus eryngii
0.293
-
3-anisyl alcohol pH 8.0, 25°C, recombinant enzyme from Emericella nidulans Pleurotus eryngii
0.41
-
veratryl alcohol pH 8.0, 25°C, wild-type enzyme Pleurotus eryngii
0.541
-
veratryl alcohol pH 8.0, 25°C, recombinant enzyme from Escherichia coli Pleurotus eryngii
0.59 1 veratryl alcohol pH 8.0, 25°C, recombinant enzyme from Emericella nidulans Pleurotus eryngii
0.758
-
benzyl alcohol pH 8.0, 25°C, recombinant enzyme from Emericella nidulans Pleurotus eryngii
0.84
-
benzyl alcohol pH 8.0, 25°C, wild-type enzyme Pleurotus eryngii
0.873
-
benzyl alcohol pH 8.0, 25°C, recombinant enzyme from Escherichia coli Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61847
-
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry Pleurotus eryngii
69114
-
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry Pleurotus eryngii
69792
-
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pleurotus eryngii the enzyme provides H2O2 for fungal degradation of lignin ?
-
?

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94219 precursor; gene aao
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG1-tagged enzyme in Escherichia coli strain W3110 solubilized from inclusion bodies 9.6fold by anion exchange chromatography Pleurotus eryngii

Renatured (Commentary)

Renatured (Comment) Organism
the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies Pleurotus eryngii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
96
-
purified recombinant enzyme from Escherichia coli Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-hexadien-1-ol + O2
-
Pleurotus eryngii 2,4-hexadienal + H2O2
-
?
3-anisyl alcohol + O2
-
Pleurotus eryngii 3-anisyl aldehyde + H2O2
-
?
4-anisyl alcohol + O2
-
Pleurotus eryngii 4-anisyl aldehyde + H2O2
-
?
benzyl alcohol + O2
-
Pleurotus eryngii benzaldehyde + H2O2
-
?
additional information the enzyme provides H2O2 for fungal degradation of lignin Pleurotus eryngii ?
-
?
additional information oxidation of aromatic and aliphatic polyunsaturated primary alcohols by wild-type and recombinant enzymes, overview Pleurotus eryngii ?
-
?
veratryl alcohol + O2
-
Pleurotus eryngii veratryl aldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry Pleurotus eryngii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
FAD a flavoenzyme Pleurotus eryngii