Cloned (Comment) | Organism |
---|---|
expression in Emericella nidulans under control of the fungal alc promoter | Pleurotus eryngii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Phenyl-1-propanol | competitive | Pleurotus eryngii | |
4-anisic acid | competitive | Pleurotus eryngii | |
4-methoxybenzylamine | uncompetitive, pH-dependent inhibition, best at pH 8.0 | Pleurotus eryngii | |
benzylmethyl ether | competitive | Pleurotus eryngii | |
chavicol | competitive | Pleurotus eryngii | |
phenol | competitive | Pleurotus eryngii | |
Toluene | competitive | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow and steady-state kinetics | Pleurotus eryngii | |
0.014 | - |
3-chloro-4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.027 | - |
4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.094 | - |
2,4-hexadien-1-ol | pH 6.0, 24°C | Pleurotus eryngii | |
0.107 | - |
3-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.132 | - |
4-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.227 | - |
3-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.54 | - |
veratryl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.553 | - |
4-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.554 | - |
3-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.632 | - |
benzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.708 | - |
cinnamyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
0.831 | - |
isovanillyl alcohol | pH 6.0, 24°C | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + O2 | Pleurotus eryngii | - |
benzaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Emericella nidulans by gel filtration and anion exchange chromatography | Pleurotus eryngii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 | the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, the enzyme does not thermodynamically stabilize a flavin semiquinone radical and forms no sulphite adduct | Pleurotus eryngii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-hexadien-1-ol + O2 | high activity | Pleurotus eryngii | 2,4-hexadienal + H2O2 | - |
? | |
3-anisyl alcohol + O2 | - |
Pleurotus eryngii | 3-anisyl aldehyde + H2O2 | - |
? | |
3-chloro-4-anisyl alcohol + O2 | high activity | Pleurotus eryngii | 3-chloro-4-anisyl aldehyde + H2O2 | - |
? | |
3-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 3-chlorobenzyl aldehyde + H2O2 | - |
? | |
3-fluorobenzyl alcohol + O2 | low activity | Pleurotus eryngii | 3-fluorobenzyl aldehyde + H2O2 | - |
? | |
4-anisyl alcohol + O2 | best substrate | Pleurotus eryngii | 4-anisyl aldehyde + H2O2 | - |
? | |
4-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-chlorobenzyl aldehyde + H2O2 | - |
? | |
4-fluorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-fluorobenzyl aldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, overview | Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
cinnamyl alcohol + O2 | - |
Pleurotus eryngii | cinnamyl aldehyde + H2O2 | - |
? | |
isovanillyl alcohol + O2 | - |
Pleurotus eryngii | isovanillyl aldehyde + H2O2 | - |
? | |
additional information | 4-(hydroxymethyl)-benzoic acid is a poor substrate | Pleurotus eryngii | ? | - |
? | |
veratryl alcohol + O2 | - |
Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Pleurotus eryngii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
90% of maximal activity within this range | Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
3-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
15 | - |
3-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
22 | - |
3-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
30 | - |
benzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
32 | - |
4-fluorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
46 | - |
3-chloro-4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
51 | - |
4-Chlorobenzyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
65 | - |
cinnamyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
114 | - |
veratryl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
119 | - |
2,4-hexadien-1-ol | pH 6.0, 24°C | Pleurotus eryngii | |
127 | - |
isovanillyl alcohol | pH 6.0, 24°C | Pleurotus eryngii | |
142 | - |
4-anisyl alcohol | pH 6.0, 24°C | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pleurotus eryngii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 11 | - |
Pleurotus eryngii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | very stable | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme, involved in the catalytic reaction | Pleurotus eryngii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetcis | Pleurotus eryngii | |
0.08 | - |
4-anisic acid | pH 6.0, 24°C | Pleurotus eryngii | |
0.11 | - |
chavicol | pH 6.0, 24°C | Pleurotus eryngii | |
0.25 | - |
4-methoxybenzylamine | pH 8.0, 24°C | Pleurotus eryngii | |
0.75 | - |
Toluene | pH 6.0, 24°C | Pleurotus eryngii | |
1.35 | - |
benzylmethyl ether | pH 6.0, 24°C | Pleurotus eryngii | |
1.92 | - |
phenol | pH 6.0, 24°C | Pleurotus eryngii | |
4.48 | - |
3-Phenyl-1-propanol | pH 6.0, 24°C | Pleurotus eryngii |