Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | activates 1.6fold at 10% | Streptomyces sp. | |
Isopropanol | activates 2.3fold at 10% | Streptomyces sp. | |
methanol | activates 1.15fold at 10% | Streptomyces sp. | |
Triton X-100 | acivates 3.5fold at 0.5% | Streptomyces sp. | |
Triton X-114 | acivates 2fold at 0.5% | Streptomyces sp. | |
Tween 80 | acivates 1.5fold at 0.5% | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Streptomyces sp. | |
acetone | inhibits 43% at 10% | Streptomyces sp. | |
Ba2+ | inhibits 21.4% at 5 mM | Streptomyces sp. | |
Benzene | inhibits 20% at 10% | Streptomyces sp. | |
chloroform | inhibits 80% at 10% | Streptomyces sp. | |
dithiothreitol | - |
Streptomyces sp. | |
DMSO | inhibits 5% at 10% | Streptomyces sp. | |
Hg2+ | inhibits 21.8% at 5 mM | Streptomyces sp. | |
Mn2+ | inhibits 4.5% at 5 mM | Streptomyces sp. | |
Sodium dodecyl sulfate | inactivates the enzyme | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1013 | - |
cholesterol | pH 7.0, 37°C | Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 3.6% at 5 mM | Streptomyces sp. | |
Cu2+ | activates 3fold at 5 mM | Streptomyces sp. | |
additional information | no or poor effects at 5 mM by Ca2+, Zn2+, Mg2+, and Fe2+ | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, SDS-PAGE | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces sp. | - |
cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Streptomyces sp. R-6 | - |
cholest-4-en-3-one + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the enzyme shows moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increases in presence of isopropanol and ethanol | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. R-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 14.3fold by ammonium sulfate fractionation and affinity chromatography | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | effect of carbon and nitrogen sources on COD production, highest activity on lactose and sucrose and on yeast extract, respectively, overview | Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.8 | - |
purified enzyme, pH 7.0, 37°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces sp. R-6 | cholest-4-en-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
COD | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, profile overview | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, profile overview | Streptomyces sp. |