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Literature summary for 1.1.3.6 extracted from

  • Niwas, R.; Singh, V.; Singh, R.; Tripathi, D.; Tripathi, C.K.
    Production, purification and characterization of cholesterol oxidase from a newly isolated Streptomyces sp. (2013), World J. Microbiol. Biotechnol., 29, 2077-2085.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ethanol activates 1.6fold at 10% Streptomyces sp.
Isopropanol activates 2.3fold at 10% Streptomyces sp.
methanol activates 1.15fold at 10% Streptomyces sp.
Triton X-100 acivates 3.5fold at 0.5% Streptomyces sp.
Triton X-114 acivates 2fold at 0.5% Streptomyces sp.
Tween 80 acivates 1.5fold at 0.5% Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Streptomyces sp.
acetone inhibits 43% at 10% Streptomyces sp.
Ba2+ inhibits 21.4% at 5 mM Streptomyces sp.
Benzene inhibits 20% at 10% Streptomyces sp.
chloroform inhibits 80% at 10% Streptomyces sp.
dithiothreitol
-
Streptomyces sp.
DMSO inhibits 5% at 10% Streptomyces sp.
Hg2+ inhibits 21.8% at 5 mM Streptomyces sp.
Mn2+ inhibits 4.5% at 5 mM Streptomyces sp.
Sodium dodecyl sulfate inactivates the enzyme Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1013
-
cholesterol pH 7.0, 37°C Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates 3.6% at 5 mM Streptomyces sp.
Cu2+ activates 3fold at 5 mM Streptomyces sp.
additional information no or poor effects at 5 mM by Ca2+, Zn2+, Mg2+, and Fe2+ Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
x * 62000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Streptomyces sp.
-
cholest-4-en-3-one + H2O2
-
?
cholesterol + O2 Streptomyces sp. R-6
-
cholest-4-en-3-one + H2O2
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme shows moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increases in presence of isopropanol and ethanol Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. R-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 14.3fold by ammonium sulfate fractionation and affinity chromatography Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information effect of carbon and nitrogen sources on COD production, highest activity on lactose and sucrose and on yeast extract, respectively, overview Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
purified enzyme, pH 7.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Streptomyces sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces sp. R-6 cholest-4-en-3-one + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
COD
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, profile overview Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, profile overview Streptomyces sp.