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Literature summary for 1.1.3.6 extracted from

  • Lin, Y.; Fu, J.; Song, X.
    Purification and characterization of an extracellular cholesterol oxidase from a Bordetella species (2010), Process Biochem., 45, 1563-1569.
No PubMed abstract available
Search for more literature for 1.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 88% inhibition at 1 mM Bordetella sp.
Hg2+ 90% inhibition at 1 mM Bordetella sp.
Na+ slight inhibition at 1 mM Bordetella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.556
-
 cholesterol pH 7.0, 37°C Bordetella sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bordetella sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates slightly at 1 mM Bordetella sp.
Ca2+ 15% activation at 1 mM Bordetella sp.
Co2+ 10% activation at 1 mM Bordetella sp.
Cu2+ activates 2.19fold at 1 mM Bordetella sp.
Fe3+ activates slightly at 1 mM Bordetella sp.
K+ activates slightly at 1 mM Bordetella sp.
Li+ activates slightly at 1 mM Bordetella sp.
Mg2+ activates slightly at 1 mM Bordetella sp.
Mn2+ 25% activation at 1 mM Bordetella sp.
Sr2+ activates slightly at 1 mM Bordetella sp.
Zn2+ activates slightly at 1 mM Bordetella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Bordetella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Bordetella sp.
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Bordetella sp. B4
-
 cholest-4-en-3-one + H2O2
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 6% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
benzene 127% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
Butanol 77% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
chloroform 96% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
cyclohexane 119% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
dimethyl sulfoxide 88% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
Ethanol 5% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
Ethyl acetate 93% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
Methanol 4% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.
xylene 93% the activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking Bordetella sp.

Organism

Organism UniProt Comment Textmining
Bordetella sp.
-
 isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China
-
Bordetella sp. B4
-
 isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from medium 12fold to homogeneity by ammonium sulfate fractionation, dialysis, ultrafiltration, anion exchange chromatography and gel filtration Bordetella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.81
-
 purified enzyme, pH 7.0, 37°C Bordetella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Bordetella sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Bordetella sp. B4 cholest-4-en-3-one + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Bordetella sp.

Synonyms

Synonyms Comment Organism
COD
-
 Bordetella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bordetella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 purified enzyme, stable at Bordetella sp.
50
-
 purified enzyme, 90% or 80% activity remaining after 1 h or 2 h, respectively Bordetella sp.
60
-
 purified enzyme, 20% activity remaining after 2 h Bordetella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bordetella sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range Bordetella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 purified enzyme, 4°C for 48 h, 80% activity remaining Bordetella sp.
5
-
 purified enzyme, 4°C for 48 h, completely stable Bordetella sp.
6 10 purified enzyme, 4°C for 48 h, 80% activity remaining Bordetella sp.