Application | Comment | Organism |
---|---|---|
analysis | the development of an enzyme-based sensor employing the enzyme ChOx has great potential as a simple and economical sensor system, engineering of the enzyme for electrochemical monitoring of cholesterol, overview | Streptomyces sp. |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes with with His-tags both at the N-terminus and at the C-terminus in Escherichia coli strain BL21 (DE3) | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
E361A | site-directed mutagenesis, the mutant shows no dehydrogenase activity | Streptomyces sp. |
F359A | site-directed mutagenesis, the dehydrogenase/oxidase ratio is 12fold increased compared with the ratio for the wild-type enzyme | Streptomyces sp. |
F444A | site-directed mutagenesis, the dehydrogenase/oxidase ratio is 4fold increased compared with the ratio for the wild-type enzyme | Streptomyces sp. |
M122A | site-directed mutagenesis, the dehydrogenase/oxidase ratio is 26fold increased compared with the ratio for the wild-type enzyme | Streptomyces sp. |
additional information | site-directed mutagenesis on oxygen-binding residues, which are observed in the high-resolution crystal structure, in order to elucidate the amino acid residues responsible for the oxidase activity, overview. Engineering of the enzyme for electrochemical monitoring of cholesterol | Streptomyces sp. |
N485A | site-directed mutagenesis, the mutant shows no dehydrogenase activity | Streptomyces sp. |
V124A | site-directed mutagenesis, the dehydrogenase/oxidase ratio is 30fold increased compared with the ratio for the wild-type enzyme | Streptomyces sp. |
V191A | site-directed mutagenesis, the mutant enzymes shows a significant decrease in its oxidase activity, but shows increased dehydrogenase activity. The dehydrogenase/oxidase ratio of Val191Ala is more than 150%, which is a 408fold increase compared with the ratio for the wild-type enzyme, substrate inhibition with cholesterol | Streptomyces sp. |
Y446A | site-directed mutagenesis, the dehydrogenase/oxidase ratio is 3fold increased compared with the ratio for the wild-type enzyme | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholesterol | substrate inhibition of mutant V191A | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00245 | - |
cholesterol | recombinant mutant V191A, pH 7.0, 22°C | Streptomyces sp. | |
0.12 | - |
cholesterol | recombinant wild-type enzyme, pH 7.0, 22°C | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces sp. | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes with with His-tags both at the N-terminus and at the C-terminus from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.049 | - |
purified recombinant mutant V191A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
0.061 | - |
purified recombinant mutant M122A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
0.074 | - |
purified recombinant mutant F359A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
0.15 | - |
purified recombinant mutant V124A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
0.22 | - |
purified recombinant mutant F444A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
0.28 | - |
purified recombinant mutant Y446A, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
2.7 | - |
purified recombinant wild-type enzyme, oxidase activity, pH 7.0, 22°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | assay with phenazine sulfate and dichlorophenylindophenol as electron acceptors | Streptomyces sp. | cholest-5-en-3-one + H2O2 | - |
? | |
additional information | the wild-type enzyme also shows cholesterol dehydrogenase activity with 0.001 U/mg | Streptomyces sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChOx | - |
Streptomyces sp. |
type I ChOx | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.21 | - |
cholesterol | recombinant mutant V191A, pH 7.0, 22°C | Streptomyces sp. | |
47.3 | - |
cholesterol | recombinant wild-type enzyme, pH 7.0, 22°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | responsible for electron transfer to O2 | Streptomyces sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
394 | - |
cholesterol | recombinant wild-type enzyme, pH 7.0, 22°C | Streptomyces sp. | |
902 | - |
cholesterol | recombinant mutant V191A, pH 7.0, 22°C | Streptomyces sp. |