Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase | Streptomyces parvus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Streptomyces parvus | |
Ag+ | - |
Streptomyces parvus | |
DTT | - |
Streptomyces parvus | |
Hg2+ | - |
Streptomyces parvus | |
Pb2+ | - |
Streptomyces parvus | |
Zn2+ | - |
Streptomyces parvus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces parvus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Streptomyces parvus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Streptomyces parvus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces parvus | - |
cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Streptomyces parvus 13647 J | - |
cholest-4-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces parvus | - |
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India) | - |
Streptomyces parvus 13647 J | - |
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India) | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 18fold by anion exchange chromatography and gel filtration | Streptomyces parvus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Streptomyces parvus shows markedly high cho activity in culture broth | Streptomyces parvus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, pH 7.2, 50°C | Streptomyces parvus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxyandrost-5-en-17-one + O2 | 30% of the activity with cholesterol | Streptomyces parvus | androst-5-en-3,17-dione + H2O2 | - |
? | |
3beta-hydroxyandrost-5-en-17-one + O2 | 30% of the activity with cholesterol | Streptomyces parvus 13647 J | androst-5-en-3,17-dione + H2O2 | - |
? | |
3beta-hydroxypregn-5-en-20-one + O2 | 42% of the activity with cholesterol | Streptomyces parvus | pregn-5-en-3,20-dione + H2O2 | - |
? | |
3beta-hydroxypregn-5-en-20-one + O2 | 42% of the activity with cholesterol | Streptomyces parvus 13647 J | pregn-5-en-3,20-dione + H2O2 | - |
? | |
5alpha-androstan-3-ol-17-one + O2 | 15% of the activity with cholesterol | Streptomyces parvus | 5alpha-androstan-3,17-dione + H2O2 | - |
? | |
5alpha-cholestan-3beta-ol + O2 | equally active as cholesterol | Streptomyces parvus | 5alpha-cholestan-3-one + H2O2 | - |
? | |
5alpha-cholestan-3beta-ol + O2 | equally active as cholesterol | Streptomyces parvus 13647 J | 5alpha-cholestan-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces parvus | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces parvus 13647 J | cholest-4-en-3-one + H2O2 | - |
? | |
ergosta-5,7,22-trien-3beta-ol + O2 | 18% of the activity with cholesterol | Streptomyces parvus | ergostan-5,7,22-trien-3-one + H2O2 | - |
? | |
additional information | no activity with cholest-5-en-3alpha-ol | Streptomyces parvus | ? | - |
? | |
additional information | no activity with cholest-5-en-3alpha-ol | Streptomyces parvus 13647 J | ? | - |
? | |
sitost-5-en-3beta-ol + O2 | 88% of the activity with cholesterol | Streptomyces parvus | sitost-5-en-3-one + H2O2 | - |
? | |
stigmast-5-en-3beta-ol + O2 | 68% of the activity with cholesterol | Streptomyces parvus | stigmast-5-en-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Streptomyces parvus |
Synonyms | Comment | Organism |
---|---|---|
CHO | - |
Streptomyces parvus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces parvus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 65 | purified enzyme, stable at | Streptomyces parvus |
55 | - |
purified enzyme, 30 min, 68% activity remaining | Streptomyces parvus |
60 | - |
purified enzyme, 30 min, 66% activity remaining | Streptomyces parvus |
65 | - |
purified enzyme, 30 min, 46% activity remaining | Streptomyces parvus |
75 | - |
purified enzyme, 30 min, 14% activity remaining | Streptomyces parvus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces parvus |