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Literature summary for 1.1.3.6 extracted from

  • Xin, Y.; Yang, H.; Xia, X.; Zhang, L.; Zhang, Y.; Tong, Y.; Chen, Y.; Wang, W.
    Expression and comparison of recombinant cholesterol oxidases (COD) in Escherichia coli with native cholesterol oxidase expressed in Brevibacterium sp (2011), Afr. J. Biotechnol., 10, 14968-14976.
No PubMed abstract available
Search for more literature for 1.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21-CodonPlus (DE3)-RP of the enzyme with and without N- or C-terminal His-tags, the hydrophobicity of the enzyme is reduced by His-tags Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24.3
-
 cholesterol native enzyme, pH 7.2, 37°C Brevibacterium sp.
25.4
-
 cholesterol non-tagged recombinant enzyme, pH 7.2, 37°C Brevibacterium sp.
35.4
-
 cholesterol N-terminally His-tagged recombinant enzyme , pH 7.2, 37°C Brevibacterium sp.
45.2
-
 cholesterol C-terminally His-tagged recombinant enzyme , pH 7.2, 37°C Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Brevibacterium sp.
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Brevibacterium sp. M201008
-
 cholest-4-en-3-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. M201008
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 C-terminally His-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C Brevibacterium sp.
12
-
 N-terminally His-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C Brevibacterium sp.
16
-
 non-tagged recombinant enzyme expressed in Escherichia coli, pH 7.2, 37°C Brevibacterium sp.
17.5
-
 native enzyme from Brevibacterium sp., pH 7.2, 37°C Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Brevibacterium sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Brevibacterium sp. M201008 cholest-4-en-3-one + H2O2
-
 ?

Subunits

Subunits Comment Organism
More native cholesterol oxidase is composed of 39% alpha-helix, 40% beta-sheet, and 20% random coil, while the non-tagged enzyme is composed of 40% alpha-helix, 35% beta-sheet, and 24% random coil. In contrast, the N-terminally His-tagged enzyme is composed of 45% alpha-helix, 29% beta-sheet, and 25% random coil, and the C-terminally His-tagged enzyme is composed of 55% alpha-helix, 16% beta-sheet, and 28% random coil Brevibacterium sp.

Synonyms

Synonyms Comment Organism
COD
-
 Brevibacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Brevibacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH-dependencies of native and recombinant non-tagged or His-tagged enzyme is similar, overview Brevibacterium sp.
5 11 activity range Brevibacterium sp.