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Literature summary for 1.1.3.6 extracted from

  • Ghasemian, A.; Yazdi, M.; Sepehrizadeh, Z.; Yazdi, Z.; Zarrini, G.
    Overexpression, one-step purification, and characterization of a type II cholesterol oxidase from a local isolate Rhodococcus sp. PTCC 1633 (2009), World J. Microbiol. Biotechnol., 25, 773-779.
No PubMed abstract available
Search for more literature for 1.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 cholesterol pH 7.0, 50°C Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. Q157H4
-
-
Rhodococcus sp. PTCC 1633 Q157H4
-
-

Purification (Commentary)

Purification (Comment) Organism
to purify the recombinant enzyme, 500 mg cholesterol is added to 50 ml of crude extract, and then incubated for 2 h at 4°C followed by centrifugation at 10000g for 10 min Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-sitosterol + O2 64% activity compared to cholesterol Rhodococcus sp. stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 64% activity compared to cholesterol Rhodococcus sp. PTCC 1633 stigmast-5-en-3-one + H2O2
-
 ?
beta-stigmasterol + O2 33% activity compared to cholesterol Rhodococcus sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 33% activity compared to cholesterol Rhodococcus sp. PTCC 1633 stigmasta-5,22-dien-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Rhodococcus sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Rhodococcus sp. PTCC 1633 cholest-4-en-3-one + H2O2
-
 ?
additional information no activity with cholic acid, progesterone and 4-androsten-3,17-dione Rhodococcus sp. ?
-
 ?
additional information no activity with cholic acid, progesterone and 4-androsten-3,17-dione Rhodococcus sp. PTCC 1633 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 after 1 h of incubation, more than 70 and 50% of activity remains at 45 and 55°C, respectively Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 relative high activities in a broad pH range of 6.0-8.0, however activity rapidly declines under pH 5.5 Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rhodococcus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus sp. pI-value about 9.0, isoelectric focusing
-
 9