Application | Comment | Organism |
---|---|---|
biotechnology | Cholesterol oxidase has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable intermediates for industrial steroid drug production | Brevibacterium sterolicum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Brevibacterium sterolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni-chelating column chromatography | Brevibacterium sterolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the type II cholesterol oxidase fully active enzyme contains covalently bound FAD | Brevibacterium sterolicum |