Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mM, 34% loss of activity | Chromobacterium sp. DS-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
cholesterol | - |
Chromobacterium sp. DS-1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Chromobacterium sp. DS-1 | - |
- |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, 90% loss of activity | Chromobacterium sp. DS-1 |
benzene | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
Butanol | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
chloroform | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
cyclooctane | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
dimethyl sulfoxide | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
Ethanol | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
Ethyl acetate | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
isopropanol | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
Methanol | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
p-xylene | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
toluene | 37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable | Chromobacterium sp. DS-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium sp. DS-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Chromobacterium sp. DS-1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Chromobacterium sp. DS-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.9 | - |
- |
Chromobacterium sp. DS-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cholestanol + O2 | 95% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
beta-sitosterol + O2 | 80% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
beta-stigmasterol + O2 | 36% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
cholesterol + O2 | - |
Chromobacterium sp. DS-1 | cholest-4-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | 27% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
epiandrosterone + O2 | 15% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
ergosterol + O2 | 12% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
additional information | no activity with epicholesterol | Chromobacterium sp. DS-1 | ? | - |
? | |
pregnenolone + O2 | 95% of the activity with cholesterol | Chromobacterium sp. DS-1 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Chromobacterium sp. DS-1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 70% of maximal activity, 80°C: about 40% of maximal activity | Chromobacterium sp. DS-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
240 min, enzyme retains 99% of its activity | Chromobacterium sp. DS-1 |
60 | - |
240 min, enzyme retains 87% of its activity | Chromobacterium sp. DS-1 |
80 | - |
60 min, the enzyme retains 90% of its activity | Chromobacterium sp. DS-1 |
85 | - |
30 min, 20% loss of activity | Chromobacterium sp. DS-1 |
90 | - |
30 min, 72% loss of activity | Chromobacterium sp. DS-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Chromobacterium sp. DS-1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6.0: about 65% of maximal activity, pH 9.5: about 60% of maximal activcity | Chromobacterium sp. DS-1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | 30°C, 1 h, stable | Chromobacterium sp. DS-1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1 mol of FAD per mole of protein | Chromobacterium sp. DS-1 |