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Literature summary for 1.1.3.6 extracted from
- Extracellular Pleurotus ostreatus cholesterol oxidase. Purification and characterization (2007), Adv. Food Sci., 29, 12-20.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 1 mM, complete inhibition | Pleurotus ostreatus |  |
| MgCl2 | 1 mM, complete inhibition | Pleurotus ostreatus | |
| p-chloromercuribenzoate | 0.01 mM, complete inhibition | Pleurotus ostreatus | |
| Pb(OAc)2 | 1 mM, complete inhibition | Pleurotus ostreatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
cholesterol | - |
Pleurotus ostreatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pleurotus ostreatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Pleurotus ostreatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pleurotus ostreatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition | the strain reaches its maximal utilization of cholesterol as the only C source for production of extracellular cholesterol oxidase | Pleurotus ostreatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | - |
Pleurotus ostreatus | cholest-4-en-3-one + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, stable | Pleurotus ostreatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pleurotus ostreatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | stable at | Pleurotus ostreatus |
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