Cloned (Comment) | Organism |
---|---|
cholesterol oxidase II, expression in Escherichia coli MM294/pnH10 | Brevibacterium sterolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | cholesterol oxidase II | Brevibacterium sterolicum | |
HgCl2 | cholesterol oxidase II | Brevibacterium sterolicum | |
PCMB | cholesterol oxidase II | Brevibacterium sterolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
cholesterol | pH 7.0, 37°C, cholesterol oxidase II | Brevibacterium sterolicum | |
1.1 | - |
cholesterol | pH 7.0, 37°C, cholesterol oxidase I | Brevibacterium sterolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46500 | - |
cholesterol oxidase II, gel filtration | Brevibacterium sterolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
nov. sp. ATCC21387 | - |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium sterolicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55.2 | - |
cholesterol oxidase II | Brevibacterium sterolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + O2 | about 30% of the activity with cholesterol, cholesterol oxidase I | Brevibacterium sterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | about 55% of the activity with cholesterol, cholesterol oxidase II | Brevibacterium sterolicum | ? | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
dehydro-epi-androsterone + O2 | about 35% of the activity with cholesterol, cholesterol oxidase I | Brevibacterium sterolicum | ? | - |
? | |
dehydro-epi-androsterone + O2 | about 5% of the activity with cholesterol, cholesterol oxidase II | Brevibacterium sterolicum | ? | - |
? | |
pregnenolone + O2 | about 60% of the activity with cholesterol, cholesterol oxidase I | Brevibacterium sterolicum | ? | - |
? | |
pregnenolone + O2 | about 60% of the activity with cholesterol, cholesterol oxidase II | Brevibacterium sterolicum | ? | - |
? | |
stigmasterol + O2 | about 10% of the activity with cholesterol, cholesterol oxidase I | Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
stigmasterol + O2 | about 10% of the activity with cholesterol, cholesterol oxidase II | Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol oxidase I | - |
Brevibacterium sterolicum |
cholesterol oxidase II | - |
Brevibacterium sterolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
cholesterol oxidase II | Brevibacterium sterolicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 1 h, 10% loss of activity | Brevibacterium sterolicum |
60 | - |
pH 7.0, 1 h, 82% loss of activity | Brevibacterium sterolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
cholesterol oxidase II | Brevibacterium sterolicum |
7 | - |
cholesterol oxidase I | Brevibacterium sterolicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 75% of maximalm acttivity, pH 8.5: about 60% of maximal activity, cholesterol oxidase II | Brevibacterium sterolicum |
5.5 | 8.5 | pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, cholesterol oxidase I | Brevibacterium sterolicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 50°C, 1 h, stable | Brevibacterium sterolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Brevibacterium sterolicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brevibacterium sterolicum | isoelectric focusing with carrier ampholites pH 4-6, cholesterol oxidase II | - |
4.9 |