Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.6 extracted from

  • Yoshimoto, T.; Ritani, A.; Ohwada, K.; Takahashi, K.; Kodera, Y.; Matsushima, A.; Saito, Y.; Inada, Y.
    Polyethylene glycol derivative-modified cholesterol oxidase soluble and active in benzene (1987), Biochem. Biophys. Res. Commun., 148, 876-882.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Nocardia sp.
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
polyethylene glycol derivative-modified enzyme Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-sitosterol + O2
-
Nocardia sp. stigmast-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia sp. cholest-5-en-3-one + H2O2
-
?
fucosterol + O2
-
Nocardia sp. stigmasta-5,24(28)-dien-3-one + H2O2
-
?