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Literature summary for 1.1.3.6 extracted from

  • Tomioka, H.; Kagawa, M.; Nakamura, S.
    Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens (1976), J. Biochem., 79, 903-915.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-Fluoro-2,4-dinitrobenzene 0.1 mM, 71% inhibition Streptomyces violascens
5alpha-Cholestan-3-one
-
Streptomyces violascens
5alpha-Cholestan-3beta,5alpha-diol
-
Streptomyces violascens
5alpha-Cholestan-3beta-ol
-
Streptomyces violascens
5alpha-Lanosta-8,24-dien-3beta-ol
-
Streptomyces violascens
5beta-Cholestan-3beta-ol
-
Streptomyces violascens
AgNO3
-
Streptomyces violascens
CuSO4 slight inhibition Streptomyces violascens
FeCl3 1.7 mM, 95% inhibition Streptomyces violascens
FeSO4 1.7 mM, 71% inhibition Streptomyces violascens
HgCl2
-
Streptomyces violascens
Iodine 0.1 mM, 91% inhibition Streptomyces violascens
KCN
-
Streptomyces violascens
additional information not inhibited by p-chloromercuribenzoate; overview: inhibition by steroids Streptomyces violascens
N-bromosuccinimide 0.01 mM, 100% inhibition Streptomyces violascens
NaN3
-
Streptomyces violascens
Trinitrobenzene sulfonate 0.3 mM, 66% inhibition Streptomyces violascens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Streptomyces violascens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Streptomyces violascens
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces violascens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces violascens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Streptomyces violascens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.7
-
-
Streptomyces violascens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Streptomyces violascens cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces violascens cholest-5-en-3-one + H2O2
-
?
dehydroepiandrosterone + O2
-
Streptomyces violascens androst-5-en-3,17-dione + H2O2
-
?
pregnenolone + O2
-
Streptomyces violascens 5-pregnen-3,20-dione + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Streptomyces violascens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 about 25% of maximal activity at 10°C and 80°C Streptomyces violascens
20 70 about 50% of maximal activity at 20°C and 70°C Streptomyces violascens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces violascens