Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Fluoro-2,4-dinitrobenzene | 0.1 mM, 71% inhibition | Streptomyces violascens | |
5alpha-Cholestan-3-one | - |
Streptomyces violascens | |
5alpha-Cholestan-3beta,5alpha-diol | - |
Streptomyces violascens | |
5alpha-Cholestan-3beta-ol | - |
Streptomyces violascens | |
5alpha-Lanosta-8,24-dien-3beta-ol | - |
Streptomyces violascens | |
5beta-Cholestan-3beta-ol | - |
Streptomyces violascens | |
AgNO3 | - |
Streptomyces violascens | |
CuSO4 | slight inhibition | Streptomyces violascens | |
FeCl3 | 1.7 mM, 95% inhibition | Streptomyces violascens | |
FeSO4 | 1.7 mM, 71% inhibition | Streptomyces violascens | |
HgCl2 | - |
Streptomyces violascens | |
Iodine | 0.1 mM, 91% inhibition | Streptomyces violascens | |
KCN | - |
Streptomyces violascens | |
additional information | not inhibited by p-chloromercuribenzoate; overview: inhibition by steroids | Streptomyces violascens | |
N-bromosuccinimide | 0.01 mM, 100% inhibition | Streptomyces violascens | |
NaN3 | - |
Streptomyces violascens | |
Trinitrobenzene sulfonate | 0.3 mM, 66% inhibition | Streptomyces violascens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Streptomyces violascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces violascens | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces violascens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces violascens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Streptomyces violascens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.7 | - |
- |
Streptomyces violascens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces violascens | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces violascens | cholest-5-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | - |
Streptomyces violascens | androst-5-en-3,17-dione + H2O2 | - |
? | |
pregnenolone + O2 | - |
Streptomyces violascens | 5-pregnen-3,20-dione + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces violascens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | about 25% of maximal activity at 10°C and 80°C | Streptomyces violascens |
20 | 70 | about 50% of maximal activity at 20°C and 70°C | Streptomyces violascens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces violascens |