Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.4 extracted from

  • Ge, J.; Jiang, X.; Liu, W.; Wang, Y.; Huang, H.; Bai, Y.; Su, X.; Yao, B.; Luo, H.
    Characterization, stability improvement, and bread baking applications of a novel cold-adapted glucose oxidase from Cladosporium neopsychrotolerans SL16 (2020), Food Chem., 310, 125970 .
    View publication on PubMed
Search for more literature for 1.1.3.4

Application

Application Comment Organism
food industry mutant enzyme Y169C/A211C is a good candidate for the bread baking industry. It has a significant effect on bread volume. Its improved thermostability, pH stability, and catalytic activity, its resistance to SDS, maximal enzymatic activity at low temperatures, and high activity under alkaline conditions are valuable properties that in addition to the bread baking industry Cladosporium neopsychrotolerans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Cladosporium neopsychrotolerans

Protein Variants

Protein Variants Comment Organism
Y169C/A211C compared with wild-type enzyme, the half-life of the mutant, at 40 °C increases approximately 48fold. The kcat and catalytic efficiency are enhanced 0.7fold and 1.6fold, respectively Cladosporium neopsychrotolerans

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 10 mM, wild-type enzyme retains approximately 80% of its activity, mutant enzyme Y169C/A211C retains 50-60% activity Cladosporium neopsychrotolerans
Mn2+ 5 mM and 10 mM, enzymatic activity of wild-type enzyme is above 100%, mutant enzyme Y169C/A211C retains 50-60% activity Cladosporium neopsychrotolerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
69
-
 beta-D-glucose pH 7.0, 20-25°C, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans
103
-
 beta-D-glucose pH 7.0, 20-25°C, wild-type enzyme Cladosporium neopsychrotolerans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 5 mM and 10 mM, enzymatic activity of wild-type enzyme is above 100% Cladosporium neopsychrotolerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64600
-
 calculated from sequence Cladosporium neopsychrotolerans
120000
-
 SDS-PAGE, wild-type and mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

Organic Solvent Stability

Organic Solvent Comment Organism
SDS wild-type and mutant enzyme Y169C/A211C are highle resistant Cladosporium neopsychrotolerans

Organism

Organism UniProt Comment Textmining
Cladosporium neopsychrotolerans A0A5Q2UVJ5
-
-
Cladosporium neopsychrotolerans SL16 A0A5Q2UVJ5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 13 putative N-glycosylation sites Cladosporium neopsychrotolerans

Purification (Commentary)

Purification (Comment) Organism
-
 Cladosporium neopsychrotolerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2
-
 Cladosporium neopsychrotolerans D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Cladosporium neopsychrotolerans SL16 D-glucono-1,5-lactone + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE, wild-type and mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

Synonyms

Synonyms Comment Organism
CngoxA
-
 Cladosporium neopsychrotolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 recombinant wild-type enzyme Cladosporium neopsychrotolerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 40 0°C: about 70% of maximal activity, 40°C: about 80% of maximal activity, recombinant wild-type enzyme Cladosporium neopsychrotolerans
-
 50 0°C: about 75% of maximal activity, 40°C: about 75% of maximal activity, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 wild-type and mutant enzyme Y169C/A211C are cold adapted Cladosporium neopsychrotolerans
30
-
 1 h, 10% loss of activity, recombinant enzyme Cladosporium neopsychrotolerans
40
-
 1 h, no loss of activity, mutant enzyme Y169C/A211C. Half-life of mutant enzyme Y169C/A211C: 720 min. Half-life of wild-type enzyme: 15 min Cladosporium neopsychrotolerans
45
-
 1 h, complete loss of activity, recombinant enzyme. 30 min, 60% loss of activity, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans
50.6
-
 Tm-value of wild-type enzyme: 50.6°C Cladosporium neopsychrotolerans
58.6
-
 Tm-value of mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
97
-
 beta-D-glucose pH 7.0, 20-25°C, wild-type enzyme Cladosporium neopsychrotolerans
164
-
 beta-D-glucose pH 7.0, 20-25°C, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 pH 7.0, 20-25°C, recombinant wild-type enzyme Cladosporium neopsychrotolerans
7.5
-
 pH 7.0, 20-25°C, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 60% of maximal activity, pH 10.0: about 40% of maximal activity, recombinant wild-type enzyme Cladosporium neopsychrotolerans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12 1 h, stable, mutant enzyme Y169C/A211C Cladosporium neopsychrotolerans
6 9 1 h, stable, recombinant enzyme Cladosporium neopsychrotolerans

pI Value

Organism Comment pI Value Maximum pI Value
Cladosporium neopsychrotolerans calculated from sequence
-
 4.66