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Literature summary for 1.1.3.39 extracted from

  • Koga, S.; Ogawa, J.; Cheng, L.Y.; Choi, Y.M.; Yamada, H.; Shimizu, S.
    Nuceloside oxidase, a hydrogen peroxide-forming oxidase, from Flavobacterium meningosepticum (1997), Appl. Environ. Microbiol., 63, 4282-4286.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.39

Inhibitors

Inhibitors Comment Organism Structure
3'-azido-3'-deoxythymidine 50% inhibition of inosine oxidation at 3.7 mM Elizabethkingia meningoseptica
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside 50% uncompetitive inhibition of inosine oxidation at 0.13 mM Elizabethkingia meningoseptica
8-hydroxyquinoline 50% inhibition at 2 mM Elizabethkingia meningoseptica
Bredinin 50% inhibition of inosine oxidation at 0.022 mM Elizabethkingia meningoseptica
Hg2+ 22% inhibition at 2 mM Elizabethkingia meningoseptica
Hydroxylamine hydrochloride 58% inhibition at 2 mM Elizabethkingia meningoseptica
iodoacetate 44% inhibition at 2 mM Elizabethkingia meningoseptica
N-ethylmaleimide 49% inhibition at 2 mM Elizabethkingia meningoseptica
NaCN 41% inhibition at 2 mM Elizabethkingia meningoseptica
NaN3 12% inhib ition at 2 mM Elizabethkingia meningoseptica
Pb2+ 90% inhibition at 2 mM Elizabethkingia meningoseptica
phenylhydrazine 92.5% inhibition at 2 mM Elizabethkingia meningoseptica
puromycin aminonucleoside 50% inhibition of inosine oxidation at 0.67 mM Elizabethkingia meningoseptica
Semicarbazide 72% inhibition at 2 mM Elizabethkingia meningoseptica
Sn2+ 94% inhibition at 2 mM Elizabethkingia meningoseptica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 2'-deoxyguanosine
-
 Elizabethkingia meningoseptica
0.02
-
 2'-deoxyadenosine
-
 Elizabethkingia meningoseptica
0.021
-
 guanosine
-
 Elizabethkingia meningoseptica
0.024
-
 adenine-9-beta-D-arabinofuranoside
-
 Elizabethkingia meningoseptica
0.035
-
 6-mercaptopurine arabinoside
-
 Elizabethkingia meningoseptica
0.048
-
 adenosine
-
 Elizabethkingia meningoseptica
0.05
-
 thymidine
-
 Elizabethkingia meningoseptica
0.05
-
 tubercidin
-
 Elizabethkingia meningoseptica
0.072
-
 Inosine
-
 Elizabethkingia meningoseptica
0.08
-
 uridine
-
 Elizabethkingia meningoseptica
0.28
-
 cytidine
-
 Elizabethkingia meningoseptica
0.32
-
 2'-deoxycytidine
-
 Elizabethkingia meningoseptica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 1 * 81000 + 1 * 69000 + 1 * 33000 + 1 * 16000, SDS-PAGE Elizabethkingia meningoseptica
33000
-
 1 * 81000 + 1 * 69000 + 1 * 33000 + 1 * 16000, SDS-PAGE Elizabethkingia meningoseptica
69000
-
 1 * 81000 + 1 * 69000 + 1 * 33000 + 1 * 16000, SDS-PAGE Elizabethkingia meningoseptica
81000
-
 1 * 81000 + 1 * 69000 + 1 * 33000 + 1 * 16000, SDS-PAGE Elizabethkingia meningoseptica
500000
-
 gel filtration Elizabethkingia meningoseptica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + 2 O2 + H2O Elizabethkingia meningoseptica
-
 2'-deoxy-9-riburonosyladenine + 2 H2O2
-
 ?
2'-deoxycytidine + 2 O2 + H2O Elizabethkingia meningoseptica 26% of the activity of adenosine 2'-deoxy-1-riburonosylcytosine + 2 H2O2
-
 ?
2'-deoxyguanosine + 2 O2 + H2O Elizabethkingia meningoseptica 40% of the activity of adenosine 2'-deoxy-9-riburonosylguanine + 2 H2O2
-
 ?
adenosine + O2 Elizabethkingia meningoseptica
-
 9-riburonosyladenine + H2O2
-
 ?
cytidine + 2 O2 + H2O Elizabethkingia meningoseptica 50% of the activity of adenosine 1-riburonosylcytosine + 2 H2O2
-
 ?
guanosine + 2 O2 + H2O Elizabethkingia meningoseptica
-
 9-riburonosylguanine + 2 H2O2
-
 ?
thymidine + 2 O2 + H2O Elizabethkingia meningoseptica
-
 1-(2-deoxyriburonosyl)thymine + 2 H2O2
-
 ?
uridine + 2 O2 + H2O Elizabethkingia meningoseptica
-
 1-riburonosyluracil + 2 H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-
Elizabethkingia meningoseptica T-2799
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information heme-containing flavoprotein, 6.5 mol protoheme/mol enzyme Elizabethkingia meningoseptica

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Elizabethkingia meningoseptica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Elizabethkingia meningoseptica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91.8
-
-
 Elizabethkingia meningoseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,N6-ethenoadenosine + O2 56% of the activity compared to adenosine Elizabethkingia meningoseptica 9-riburonosyl-1,N6-ethenoadenine + H2O2
-
 ?
2'-deoxy-1-methyladenosine hydrochloride + O2
-
 Elizabethkingia meningoseptica 2'-deoxy-9-riburonosyl-1-methyladenine hydrochloride + H2O2
-
 ?
2'-deoxyadenosine + 2 O2 + H2O
-
 Elizabethkingia meningoseptica 2'-deoxy-9-riburonosyladenine + 2 H2O2
-
 ?
2'-deoxycytidine + 2 O2 + H2O 26% of the activity of adenosine Elizabethkingia meningoseptica 2'-deoxy-1-riburonosylcytosine + 2 H2O2
-
 ?
2'-deoxyguanosine + 2 O2 + H2O 40% of the activity of adenosine Elizabethkingia meningoseptica 2'-deoxy-9-riburonosylguanine + 2 H2O2
-
 ?
2'-tosyladenosine + O2 low activity Elizabethkingia meningoseptica 2'-tosyl-9-riburonosyladenine + H2O2
-
 ?
6-(gamma,gamma-dimethylallylamino)purine riboside + O2
-
 Elizabethkingia meningoseptica riburonosyl-6-(gamma,gamma-dimethylallylamino)purine + H2O2
-
 ?
6-benzylaminopurine riboside + O2
-
 Elizabethkingia meningoseptica riburonosyl-6-benzylaminopurine + H2O2
-
 ?
6-mercaptopurine arabinoside + O2 40% of the activity compared to adenosine Elizabethkingia meningoseptica arabinorosyl 6-mercaptopurine + H2O2
-
 ?
6-mercaptopurine arabinoside + O2 40% of the activity compared to adenosine Elizabethkingia meningoseptica T-2799 arabinorosyl 6-mercaptopurine + H2O2
-
 ?
6-mercaptopurine riboside + O2
-
 Elizabethkingia meningoseptica riburonosyl-6-mercaptopurine + H2O2
-
 ?
8-bromoadenosine + O2 49% of the activity compared to adenosine Elizabethkingia meningoseptica 8-bromo-9-riburonosyladenine + H2O2
-
 ?
adenine-9-beta-D-arabinofuranoside + O2 60% of the activity compared to adenosine Elizabethkingia meningoseptica 9-beta-D-arabinofuranuronosyladenine + H2O2
-
 ?
adenine-9-beta-D-arabinofuranoside + O2 60% activity compared to adenosine Elizabethkingia meningoseptica 9-beta-D-arabinofuranuronosyladenine + H2O2
-
 r
adenine-9-beta-D-arabinofuranoside + O2 60% of the activity compared to adenosine Elizabethkingia meningoseptica T-2799 9-beta-D-arabinofuranuronosyladenine + H2O2
-
 ?
adenine-9-beta-D-arabinofuranoside + O2 60% activity compared to adenosine Elizabethkingia meningoseptica T-2799 9-beta-D-arabinofuranuronosyladenine + H2O2
-
 r
adenosine + O2
-
 Elizabethkingia meningoseptica 9-riburonosyladenine + H2O2
-
 ?
adenosine-N'-oxide + O2
-
 Elizabethkingia meningoseptica riburonosyl-adenine-N'-oxide + H2O2
-
 ?
cytidine + 2 O2 + H2O 50% of the activity of adenosine Elizabethkingia meningoseptica 1-riburonosylcytosine + 2 H2O2
-
 ?
formycin B + O2
-
 Elizabethkingia meningoseptica (5S)-2,5-anhydro-5-(7-oxo-4,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-L-ribonic acid + H2O2
-
 ?
guanine-9-beta-D-arabinofuranoside + O2 70% of the activity compared to adenosine Elizabethkingia meningoseptica 9-beta-D-arabinofuranuronosylguanine + H2O2
-
 ?
guanine-9-beta-D-arabinofuranoside + O2 70% of the activity compared to adenosine Elizabethkingia meningoseptica T-2799 9-beta-D-arabinofuranuronosylguanine + H2O2
-
 ?
guanosine + 2 O2 + H2O
-
 Elizabethkingia meningoseptica 9-riburonosylguanine + 2 H2O2
-
 ?
hypoxanthine-9-beta-D-arabinofuranoside + O2 49% of the activity compared to adenosine Elizabethkingia meningoseptica 9-beta-D-arabinofuranuronosylhypoxanthine + H2O2
-
 ?
hypoxanthine-9-beta-D-arabinofuranoside + O2 49% of the activity compared to adenosine Elizabethkingia meningoseptica T-2799 9-beta-D-arabinofuranuronosylhypoxanthine + H2O2
-
 ?
inosine + 2 O2 + H2O
-
 Elizabethkingia meningoseptica 9-riburonosylinosine + 2 H2O2
-
 ?
kinetin riboside + O2
-
 Elizabethkingia meningoseptica D-ribofuranuronosylkinetin + H2O2
-
 ?
N-benzoyl-2'-deoxyadenosine + O2
-
 Elizabethkingia meningoseptica 9-riburonosyl-N-benzoyl-2'-deoxyadenosine + H2O2
-
 ?
ribavirin + O2 low activity Elizabethkingia meningoseptica 1-(beta-D-ribofuranuronosyl)-1H-1,2,4-triazole-3-carboxamide + H2O2
-
 ?
thymidine + 2 O2 + H2O
-
 Elizabethkingia meningoseptica 1-(2-deoxyriburonosyl)thymine + 2 H2O2
-
 ?
tubercidin + O2
-
 Elizabethkingia meningoseptica 7-(beta-D-ribo-pentodialdo-1,4-furanosyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine + H2O2
-
 ?
uridine + 2 O2 + H2O
-
 Elizabethkingia meningoseptica 1-riburonosyluracil + 2 H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 81000 + 1 * 69000 + 1 * 33000 + 1 * 16000, SDS-PAGE Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 oxidation of inosine Elizabethkingia meningoseptica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable below Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 oxidation of inosine Elizabethkingia meningoseptica

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 stable within Elizabethkingia meningoseptica

Cofactor

Cofactor Comment Organism Structure
FAD 3 mol FAD/mol enzyme, covalently bound Elizabethkingia meningoseptica