Crystallization (Comment) | Organism |
---|---|
crystals of mutant enzyme D170S/T457E are grown by using the hanging-drop vapor diffusion method | Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°Cm wild-type enzyme | Penicillium simplicissimum | |
65 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170A/T457E | Penicillium simplicissimum | |
168 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme T457E | Penicillium simplicissimum | |
238 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170S/T457E | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | P56216 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(methoxymethyl)phenol + O2 + H2O | - |
Penicillium simplicissimum | 4-hydroxybenzaldehyde + methanol + H2O2 | like native enzyme the mutant enzymes T457E, D170A and d170S preferentially form the (R)-enantiomer. The mutant enzymes D170A/T457E and D170S/T457E exhibit an inverted stereospecificity | ? | |
4-ethylphenol + O2 + H2O | - |
Penicillium simplicissimum | (R)-1-(4'-hydroxyphenyl)ethanol + H2O2 | predominant product of wild-type enzyme and of mutant T457E | ir | |
4-ethylphenol + O2 + H2O | - |
Penicillium simplicissimum | (S)-1-(4'-hydroxyphenyl)ethanol + H2O2 | predominant product of mutant D170S/T457E | ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170A/T457E | Penicillium simplicissimum | |
0.05 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170S/T457E | Penicillium simplicissimum | |
1.3 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme T457E | Penicillium simplicissimum | |
3.1 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°Cm wild-type enzyme | Penicillium simplicissimum |