Protein Variants | Comment | Organism |
---|---|---|
D170E | substrate preference is similar to wild-type enzyme, as the wild-type enzyme the mutant enzyme favors the production of alkenes | Penicillium simplicissimum |
D170S | most active with branched-chain 4-alkylphenol, mutant enzyme favors the formation of alcohols | Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
4-butylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.01 | - |
4-n-Propylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.026 | - |
4-isopropylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.048 | - |
4-Ethylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.062 | - |
4-sec-butylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.075 | - |
4-sec-butylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.088 | - |
4-isopropylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-butylphenol + O2 | - |
Penicillium simplicissimum | 4-but-1-en-1-ylphenol + 4-[(1R)-1-hydroxybutyl]phenol | mutant D170E: 0% alcohol + 100% alkene. Mutant D170S: 82% alcohol + 18% alkene | ? | |
4-ethylphenol + O2 + H2O | - |
Penicillium simplicissimum | (R)-1-(4'-hydroxyphenyl)ethanol + 4-vinylphenol + H2O2 | product formed by wild-type enzyme and mutant enzyme D170S. The hydroxylation reaction in D170S is nearly completely blocked and this mutant converts short-chain alkylphenols to the corresponding alkenes. Mutant D170E: 8% alcohol + 92% alkene. Mutant D170S: 92% alcohol + 8% alkene | ? | |
4-isopropylphenol + O2 | - |
Penicillium simplicissimum | 4-isopropenylphenol + ? | mutant D170E: 8% alcohol + 92% alkene. Mutant D170S: 80% alcohol + 20% alkene | ? | |
4-n-propylphenol + O2 | - |
Penicillium simplicissimum | 1-(4'-hydroxyphenyl)propanol + 4-(prop-1-en-1-yl)phenol | mutant D170E: 7% alcohol + 93% alkene. Mutant D170S: 96% alcohol + 4% alkene | ? | |
4-Propylphenol + O2 | - |
Penicillium simplicissimum | 1-(4'-Hydroxyphenyl)propanol + 4-propenylphenol + 1-(4'-hydroxyphenyl)propanone | - |
r | |
4-sec-butylphenol + O2 | - |
Penicillium simplicissimum | ? | mutant D170E: 1% alcohol + 99% alkene. Mutant D170S: 78% alcohol + 22% alkene | ? | |
p-cresol + O2 | - |
Penicillium simplicissimum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VAO | - |
Penicillium simplicissimum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
4-Ethylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.0001 | - |
4-butylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.0002 | - |
p-Cresol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.0002 | - |
4-n-Propylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.05 | - |
4-sec-butylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.09 | - |
4-sec-butylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.12 | - |
4-butylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.13 | - |
4-isopropylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.17 | - |
4-Ethylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.18 | - |
4-isopropylphenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.26 | - |
propylphenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum |