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Literature summary for 1.1.3.21 extracted from

  • Esders, T.W.; Michrina, C.A.
    Purification and properties of L-alpha-glycerophosphate oxidase from Streptococcus faecium ATCC 12755 (1979), J. Biol. Chem., 254, 2710-2715.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information increasing the buffer concentration from 10 mM to 100 mM leads to an 2.6-10fold increase in activity, this effect is observed with potassium phosphate, sodium acetate, sodium maleate or other anionic buffers Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
fructose-1-phosphate
-
Enterococcus faecium
fructose-6-phosphate
-
Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26
-
sn-glycerol-3-phosphate
-
Enterococcus faecium

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 10 mM, 7.7fold activation Enterococcus faecium
CoCl2 10 mM, 10.2fold activation Enterococcus faecium
KCl 100 mM, 5.8fold activation Enterococcus faecium
MgCl2 10 mM, 6.8fold activation Enterococcus faecium
MnCl2 10 mM, 7.6 fold activation Enterococcus faecium
NaCl 100 mM, 3.8fold activation Enterococcus faecium
ZnCl2 1 mM, 6.3fold activation Enterococcus faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
2 * 72000, SDS-PAGE Enterococcus faecium
131000
-
strain ATCC 12755, gel filtration, sucrose density centrifugation Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol-3-phosphate + O2 Enterococcus faecium
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
ATCC 12755
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterococcus faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85 95 strain ATCC 12755 Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol-3-phosphate + O2
-
Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 high specificity for L-alpha-glycerophosphate Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Subunits

Subunits Comment Organism
dimer 2 * 72000, SDS-PAGE Enterococcus faecium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Enterococcus faecium

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group Enterococcus faecium
FAD 2 mol bound per mol of enzyme Enterococcus faecium