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Literature summary for 1.1.3.17 extracted from
- Contribution of flavin covalent linkage with histidine 99 to the reaction catalyzed by choline oxidase (2009), J. Biol. Chem., 284, 16990-16997.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS | Arthrobacter globiformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H99N | mutant, analysis of kinetic parameters | Arthrobacter globiformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | analysis of substrate kinetic isotope effects | Arthrobacter globiformis | |
| 1.3 | - |
choline | substrate choline, pH 7, 25°C | Arthrobacter globiformis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter globiformis | Q7X2H8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified with the bound flavin cofactor in the fully oxidized state | Arthrobacter globiformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
| 0.28 | - |
apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| choline oxidase | - |
Arthrobacter globiformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
analysis of temperature effect on rate constant for flavin reduction | Arthrobacter globiformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.03 | - |
choline | apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis | |
| 1.86 | - |
choline | H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | H99N mutant | Arthrobacter globiformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | H99N mutant, lower KM compared to pH 8-11. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
| 8 | 11 | H99N mutant, no impact on KM, highest KM. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arthrobacter globiformis | |
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Release 2023.1 (January 2023)
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