Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, plant expression vector pGAH/codA,codA gene and nos terminator fragments excised from pGAH/codA, ligated to stress-inducible SWPA2 promoter, fused into pCAMBIA3300 binary vector, resulting pScodA transformed into Solanum tuberosum by using Agrobacterium tumefaciens strain AGL0 | Solanum tuberosum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | glycine betaine production in transgenic plants | Solanum tuberosum | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | Solanum tuberosum | - |
betaine aldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
cv. Superior, wild-type and transgenic plants expressing bacterial choline oxidase (codA) gene of Arthrobacter globiformis | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | transgenic and wild-type | Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
transgenic plants under the control of the stress-inducible SWPA2 promoter generated, RT-PCR analysis under stress-inducing conditions, glycine betaine accumulation, oxidative stress tolerance, salt stress tolerance and drought stress tolerance of transgenic plants | Solanum tuberosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | - |
Solanum tuberosum | betaine aldehyde + H2O2 | - |
? | |
choline + O2 | oxidation of choline to glycine betaine via the intermediate betaine aldehyde, glycine betaine production in chloroplasts of transgenic plants, stress-induced expression analysis | Solanum tuberosum | betaine aldehyde + H2O2 | - |
? |