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Literature summary for 1.1.3.17 extracted from

  • Fan, F.; Gadda, G.
    On the catalytic mechanism of choline oxidase (2005), J. Am. Chem. Soc., 127, 2067-2074.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow kinetics Arthrobacter globiformis
0.25
-
choline pH 10, 25°C Arthrobacter globiformis
0.69
-
O2 pH 10, 25°C, cosubstrate choline Arthrobacter globiformis
2.54
-
O2 pH 10, 25°C, cosubstrate betaine aldehyde Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline + O2 Arthrobacter globiformis FAD-linked reaction betaine aldehyde + H2O2
-
?
FADH2 + O2 Arthrobacter globiformis
-
FAD + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
strain ATCC 8010
-

Reaction

Reaction Comment Organism Reaction ID
choline + O2 = betaine aldehyde + H2O2 choline hydroxyl proton is not in flight in the transition state for CH bond cleavage, steps of flavin reduction by choline and betaine aldehyde is rate rate limiting for the overall turnover Arthrobacter globiformis
choline + O2 = betaine aldehyde + H2O2 reaction mechanism via FAD, the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps, hydride transfer and oxygen radical mechanisms, overview Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + O2 + H2O
-
Arthrobacter globiformis betaine + H2O2
-
?
choline + O2
-
Arthrobacter globiformis betaine aldehyde + H2O2
-
?
choline + O2 FAD-linked reaction Arthrobacter globiformis betaine aldehyde + H2O2
-
?
FADH2 + O2
-
Arthrobacter globiformis FAD + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
choline pH 10, 25°C Arthrobacter globiformis
133
-
Betaine aldehyde pH 10, 25°C Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Arthrobacter globiformis