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Literature summary for 1.1.3.16 extracted from

  • Sun, W.; Shen, Y.H.; Qi, D.W.; Xiang, Z.H.; Zhang, Z.
    Molecular cloning and characterization of ecdysone oxidase and 3-dehydroecdysone-3beta-reductase involved in the ecdysone inactivation pathway of silkworm, Bombyx mori (2012), Int. J. Biol. Sci., 8, 125-138.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 x * 72000, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + O2 Bombyx mori inactivation of ecdysone 3-dehydroecdysone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori H6AGY0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
midgut also in midgut goblet cell cavities Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ecdysone + O2
-
 Bombyx mori 3-dehydroecdysone + H2O2
-
 ?
ecdysone + O2 inactivation of ecdysone Bombyx mori 3-dehydroecdysone + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE Bombyx mori

General Information

General Information Comment Organism
metabolism ecdysone is inactivated to 3-dehydroecdysone under ecdysone oxidase, followed by NAD(P)H-dependent irreversible reduction to 3-epiecdysteroid under 3-dehydroecdysone 3alpha-reductase. On the other hand, 3-dehydroecdysone undergoes reversible reduction to ecdysone by 3-dehydroecdysone 3beta-reductase in the hemolymph Bombyx mori