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Literature summary for 1.1.3.15 extracted from
- Cloning and coexpression of recombinant N-demethylase B and glycolate oxidase genes in Escherichia coli (2019), Mol. Biol. Rep., 46, 505-510 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | coexpression of N-demethylase NdmB gene from Pseudomonas putida CBB5 and glycolate oxidase gene in Escherichia coli. By two-step purification of Ni affinity chromatography and Q-Sepharose chromatography, the coexpressed NdmB and GO are separated and result in a 15.8fold purification with 8.7% yield and 12.8fold purification with 7.2% yield, respectively | Spinacia oleracea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Spinacia oleracea | 5777 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | P05414 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, Ni affinity chromatography | Spinacia oleracea |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Spinacia oleracea |
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