Literature summary for 1.1.3.15 extracted from
Lowinsohn, D.; Bertotti, M.
A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples (2008), J. Braz. Chem. Soc., 19, 637-642.
No PubMed abstract available
Application
Application |
Comment |
Organism |
diagnostics |
lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9 |
Pediococcus sp. |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
lactate + O2 |
Pediococcus sp. |
lactate detection in beer samples |
pyruvate + H2O2 |
H2O2 oxidizes Prussian Blue on an electrode, the concomitant electron flow is measured |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Pediococcus sp. |
- |
- |
- |
Storage Stability
Storage Stability |
Organism |
below 0°C, 0.1 mol/l phosphate buffer, pH 7.2 (potassium and sodium salts), 2.2 mg lactate oxidase in 0.5 ml buffer, enzymatic activity stable for several weeks |
Pediococcus sp. |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
lactate + O2 |
lactate detection in beer samples |
Pediococcus sp. |
pyruvate + H2O2 |
H2O2 oxidizes Prussian Blue on an electrode, the concomitant electron flow is measured |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
lactate oxidase |
- |
Pediococcus sp. |
LctO |
- |
Pediococcus sp. |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
6.9 |
- |
phosphate buffer, optimal operating conditions |
Pediococcus sp. |