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Literature summary for 1.1.3.15 extracted from
- Involvement of ionizable groups in catalysis of human liver glycolate oxidase (2009), J. Biol. Chem., 284, 31214-31222.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | in humans the enzyme is a potential drug target for treatment of primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H260A | site-directed mutagenesis | Homo sapiens |
| H260Q | site-directed mutagenesis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloride | inhibits the enzyme at high concentrations | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stopped-flow, steady-state, and presteady-state kinetics at different pH values, pH profiles, detailed overview | Homo sapiens | |
| 0.2 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 0.23 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 0.44 | - |
O2 | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 0.59 | - |
O2 | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 0.64 | - |
O2 | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycolate + O2 | Homo sapiens | - |
glyoxylate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UJM8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme by nickel affinity chromatography, ammonium sulfate fractionation and dialysis | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 | the pH affects the kinetic steps of the catalytic mechanism of human glycolate oxidase, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview. Formation of the enzyme-substrate complex suggests the presence of a protonated group participating in substrate binding | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycolate + O2 | - |
Homo sapiens | glyoxylate + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycolate oxidase | - |
Homo sapiens |
| GOX | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | a second-order rate constant for the capture of glycolate (kcat/Kglycolate) of 68300 M/s, and a second-order rate constant for reaction of the reduced flavin with oxygen (kcat/Koxygen) of 24500 Ms | Homo sapiens | |
| 15.7 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 20 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 31.2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
| 54.1 | - |
flavin | pH 7.0, 30°C, flavin reduction | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH profiles, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | dependent on | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 280 | - |
chloride | pH 7.0, 25°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the enzyme is involved in primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |
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