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Literature summary for 1.1.3.15 extracted from

  • Nawawi, R.A.; Baiano, J.C.; Kvennefors, E.C.; Barnes, A.C.
    Host-directed evolution of a novel lactate oxidase in Streptococcus iniae isolates from barramundi (Lates calcarifer) (2009), Appl. Environ. Microbiol., 75, 2908-2919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.15

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification, expression in Escherichia coli BL21 Star Streptococcus iniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42380
-
 calculated, 390 amino acids, 869 bp Streptococcus iniae
42384
-
 1 * 42384, type 1 enzyme Streptococcus iniae
44038
-
 1 * 44038, type 2 enzyme Streptococcus iniae
44040
-
 calculated, 407 amino acids, 920 bp, 51 bp repeat sequence compared to type 1 enzyme Streptococcus iniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactate + O2 Streptococcus iniae
-
 pyruvate + H2O2
-
 ?
lactate + O2 Streptococcus iniae QMA0177
-
 pyruvate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus iniae A9QH69 strains QMA0165 (type 1 enzyme)
-
Streptococcus iniae A9QH71 type 2 lactate oxidase isolated from Strptococcus iniae; major pathogen of farmed fish, type 2 is an isolate from diseased barramundi (Lates calcarifer) in Northern Territory, Australia
-
Streptococcus iniae QMA0177 A9QH71 type 2 lactate oxidase isolated from Strptococcus iniae; major pathogen of farmed fish, type 2 is an isolate from diseased barramundi (Lates calcarifer) in Northern Territory, Australia
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation, washing with 10 mM potassium phosphate buffer, pH 6.5, stored at -20°C, resuspended with same buffer, treated with lysozyme followed by disruption with glass beads in a bead mill, centrifugation, supernatants used for enzyme assays Streptococcus iniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.07
-
 type 1 enzyme expressed in Escherichia coli BL21Star, 10 mM potassium phophate, pH 6,5, 0.01 mM flavin adenine nucleotide, 37°C, 0.02% 2,2-azinobis (3-ethylbenzthiazolinesulfonic acid), 10 mM L-(+)-lactate, 0.5 U horseradish peroxidase (peroxidase-coupled spectrophotometric assay) Streptococcus iniae
45.76
-
 type 2 enzyme expressed in Escherichia coli BL21Star, 10 mM potassium phophate, pH 6.5, 0.01 mM flavin adenine nucleotide, 37°C, 0.02% 2,2-azinobis (3-ethylbenzthiazolinesulfonic acid), 10 mM L-(+)-lactate, 0.5 U horseradish peroxidase (peroxidase-coupled spectrophotometric assay) Streptococcus iniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactate + O2
-
 Streptococcus iniae pyruvate + H2O2
-
 ?
lactate + O2
-
 Streptococcus iniae QMA0177 pyruvate + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42384, type 1 enzyme Streptococcus iniae
monomer 1 * 44038, type 2 enzyme Streptococcus iniae

Synonyms

Synonyms Comment Organism
lactate oxidase flavin mononucleotide-dependent Streptococcus iniae
LctO type 1 enzyme Streptococcus iniae
LctO type 2 enzyme (novel variant of lactate oxidase gene) Streptococcus iniae