Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, 0.005 ml of protein solution containing 10 mg/ml protein in 0.1 M Tris, pH 7.5, mixed with the same volume of reservoir solution containing 0.4 M sodium acetate and 0.2 M sodium citrate, pH 6.5, equilibration at 4°C, soaking of crystals in 25% glycerol-containing reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Rattus norvegicus | |
0.046 | - |
L-2-hydroxy octanoate | - |
Rattus norvegicus | |
1.36 | - |
L-2-hydroxy palmitate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus norvegicus | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q07523 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 | the catalytic residue is Phe23, reaction mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-hydroxy octanoate + O2 | - |
Rattus norvegicus | 2-oxo-octanoate + H2O2 | - |
? | |
L-2-hydroxy palmitate + O2 | - |
Rattus norvegicus | 2-oxo-palmitate + H2O2 | - |
? | |
L-lactate + O2 | - |
Rattus norvegicus | pyruvate + H2O2 | - |
? | |
mandelate + O2 | oxidation of an L-2-hydroxy acid to a 2-oxoacid, model for the binding of L-mandelate into the active site, overview | Rattus norvegicus | phenylpyruvate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis, modelling, the enzyme shows the same beta8alpha8 TIM barrel structure as other structurally characterized family members of the FMN-dependent enzyme family | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
LCHAO | - |
Rattus norvegicus |
long chain hydroxy acid oxidase | - |
Rattus norvegicus |
More | the enzyme belongs to the FMN-dependent enzyme family | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
L-2-hydroxy palmitate | - |
Rattus norvegicus | |
0.99 | - |
L-2-hydroxy octanoate | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | dependent on | Rattus norvegicus |