Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight activation for short and long chain oxidases | Sus scrofa |
General Stability | Organism |
---|---|
long chain oxidase stable at -20°C, short chain oxidase stable at -20°C for several months | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxybutyrate | long chain oxidase, 13-17% inhibition at 17 mM | Sus scrofa | |
4-chloromercuribenzoate | long chain oxidase, 29-42% inhibition at 0.001 mM, short chain oxidase: 70-75% inhibition at 0.001 mM | Sus scrofa | |
Atebrin | long chain oxidase, 72-76% inhibition at 1 mM, short chain oxidase: 68-76% inhibition at 1 mM | Sus scrofa | |
Cu2+ | short chain oxidase: 86% inhibition at 0.1 mM | Sus scrofa | |
DL-beta-Phenyllactate | short chain oxidase: 10% inhibition of glycolate oxidation, 81% inhibition of L-2-hydroxisocaproate oxidation at 10 mM | Sus scrofa | |
DL-Lipoate | long chain oxidase, 35% inhibition at 0.24 mM, short chain oxidase: 46-52% inhibition at 0.01 mM | Sus scrofa | |
iodoacetate | long chain oxidase, 31-36% inhibition at 0.1 mM, short chain oxidase: no inhibition | Sus scrofa | |
L-leucine | 9-12% inhibition at 33 mM | Sus scrofa | |
L-phenylalanine | long chain oxidase, 43% inhibition at 33 mM | Sus scrofa | |
o-Iodosobenzoate | long chain oxidase, 95-100% inhibition at 0.1 mM, short chain oxidase: no inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
glycolate | short chain oxidase | Sus scrofa | |
0.68 | - |
L-2-Hydroxyisocaproate | short chain oxidase | Sus scrofa | |
0.68 | - |
L-2-Hydroxyisocaproate | short chain oxidase, at pH 6.0 and 37°C | Sus scrofa | |
2.2 | - |
L-beta-Phenyllactate | long chain oxidase | Sus scrofa | |
2.4 | - |
L-2-hydroxy-beta-methylvalerate | long chain oxidase | Sus scrofa | |
2.5 | - |
L-2-Hydroxyisocaproate | long chain oxidase | Sus scrofa | |
2.5 | - |
L-2-Hydroxyisocaproate | long chain oxidase, at pH 6.0 and 37°C | Sus scrofa | |
16 | - |
L-lactate | short chain oxidase | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
mitochondrion | - |
Sus scrofa | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, 2 distinct forms | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | two forms: long chain oxidase, short chain oxidase | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, low activity for short chain oxidase | Sus scrofa | 2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol | low activity for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol | low activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxocaproate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, traces of activity for short chain oxidase | Sus scrofa | 2-oxodecanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 2-oxooctanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol | low activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxovalerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-3-indolelactate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 3-indolepyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-beta-phenyllactate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-mandelate + 2,6-dichlorophenolindophenol | low activity | Sus scrofa | oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | (4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | highest activity for short chain oxidase, no activity for long chain oxidase | Sus scrofa | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-2-hydroxyisocaproate + O2 | 100% activity with long chain oxidase, 32% activity with short chain oxidase | Sus scrofa | 4-methyl-2-oxopentanoate + H2O2 | - |
r | |
L-lactate + 2,6-dichlorophenolindophenol | very low activity | Sus scrofa | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-phenyllactate + 2,6-dichlorophenolindophenol | - |
Sus scrofa | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
short chain oxidase | Sus scrofa |
7.9 | - |
long chain oxidase | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
Phenyllactate | competitive | Sus scrofa |