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Literature summary for 1.1.3.15 extracted from

  • Dupuis, L.; De Caro, J.; Brachet, P.; Puigserver, A.
    Purification and some characteristics of chicken liver L-2-hydroxyacid oxidase A (1990), FEBS Lett., 266, 183-186.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
glycolate
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Gallus gallus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
4 * 37800, SDS-PAGE Gallus gallus
169000
-
non-denaturating gel electrophoresis Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + O2 Gallus gallus
-
glyoxylate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
chicken
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.08
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
4°C, 10 mM Tris-HCl, 15 mM NaCl, 0.02 mM FMN, 1 mM EDTA, several weeks, cannot be stored frozen at -20°C or -80°C Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + O2
-
Gallus gallus glyoxylate + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 37800, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
L-2-hydroxyacid oxidase A
-
Gallus gallus

Cofactor

Cofactor Comment Organism Structure
FMN
-
Gallus gallus