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Literature summary for 1.1.3.13 extracted from

  • Matsumura, K.; Yamada, M.; Yamashita, T.; Muto, H.; Nishiyama, K.I.; Shimoi, H.; Isobe, K.
    Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway (2019), J. Biosci. Bioeng., 128, 13-21 .
    View publication on PubMed
Search for more literature for 1.1.3.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The OcAOD productivity of the recombinant E. coli is 24fold higher than that of Ochrobactrum sp. AIU 033, and it is further enhanced by 1.2 times by the coexpression of additional tatABC from E. coli BL21 Ochrobactrum sp. AIU 033

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm recombinant protein in Escherichia coli Ochrobactrum sp. AIU 033
-
-

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp. AIU 033
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2
-
 Ochrobactrum sp. AIU 033 butanal + H2O2
-
 ?
1-hexanol + O2
-
 Ochrobactrum sp. AIU 033 hexanal + H2O2
-
 ?
1-octanol + O2
-
 Ochrobactrum sp. AIU 033 octanal + H2O2
-
 ?
1-pentanol + O2
-
 Ochrobactrum sp. AIU 033 pentanal + H2O2
-
 ?
1-propanol + O2
-
 Ochrobactrum sp. AIU 033 propanal + H2O2
-
 ?
ethanol + O2
-
 Ochrobactrum sp. AIU 033 acetaldehyde + H2O2
-
 ?
additional information enzyme also catalyzes the oxidation of glycolate to glyoxylate, reaction of EC 1.1.3.15. No substrate: glyoxylate Ochrobactrum sp. AIU 033 ?
-
-

Subunits

Subunits Comment Organism
? x * 52000, alpha-subunit, x * 14000, beta-subunit, SDS-PAGE Ochrobactrum sp. AIU 033

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 20 min, more than 80% residual activity, recombinant protein Ochrobactrum sp. AIU 033
50
-
 20 min, more than 40% residual activity for recombinant protein, complete loss of activity for native protein Ochrobactrum sp. AIU 033

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 20 min, 25°C, more than 80% residual activity, recombinant protein Ochrobactrum sp. AIU 033