Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | complete inhibition by 50% DMSO | Aspergillus terreus | |
additional information | no inhibition by ethylene glycol, even at high concentrations | Aspergillus terreus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Aspergillus terreus | 16020 | - |
microsome | - |
Aspergillus terreus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
269000 | - |
native enzyme, gel filtration | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Aspergillus terreus MTCC 6324 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by microsomal membrane preparation by differential centrifugation. The enzyme is solubilized from the microsomal membrane using 0.5% (v/v) 3-[(3-cholamidopropyl) dimethyl ammonio] propane-1-sulfonic acid, i.e. CHAPS, and further purified by ammonium sulfate fractionation followed by anion exchange chromatography | Aspergillus terreus |
Renatured (Comment) | Organism |
---|---|
enzyme dissociation by 2 M KBr in 0.25 M potassium phosphate, pH 7.5, 0.3 mM EDTA, 5 mM 2-mercaptoethanol and 20% glycerol, for 4 days at 4°C. The inactive dissociated enzyme protein is renaturated, but still catalytically inactive, by assembly after removal of 2-mercaptoethanol and incubation with cofactor FAD. The enzyme activity is regained by incubation with the substrate. The dissociation process traverses through two FAD-associated intermediate proteins, one of whhich shows the enzyme activity. The catalytic reactivation is a slow process. Dynamic light scattering analysis, overview. DMSO and ethylene glycol are ineffective in dissociating this alcohol oxidase | Aspergillus terreus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of native, intermediate dissociated protein, and re-associated protein, overview. The re-associated enzyme protein shows poor activity with substrate (R)-2-octanol | Aspergillus terreus |
37 | - |
native intermediate dissociated enzyme protein, substrate (R)-2-octanol | Aspergillus terreus |
42 | - |
re-associated enzyme protein, substrate n-dodecanol | Aspergillus terreus |
45 | - |
re-associated enzyme protein, substrate phenyl-3-propanol | Aspergillus terreus |
49 | - |
native intermediate dissociated enzyme protein, substrate n-dodecanol | Aspergillus terreus |
62 | - |
native enzyme, substrate n-dodecanol | Aspergillus terreus |
63 | - |
native enzyme, substrate (R)-2-octanol | Aspergillus terreus |
96 | - |
re-associated enzyme protein, substrate n-heptanol | Aspergillus terreus |
183 | - |
native intermediate dissociated enzyme protein, substrate phenyl-3-propanol | Aspergillus terreus |
190 | - |
native intermediate dissociated enzyme protein, substrate n-heptanol | Aspergillus terreus |
250 | - |
native enzyme, substrate phenyl-3-propanol | Aspergillus terreus |
305 | - |
native enzyme, substrate n-heptanol | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-octanol + O2 | - |
Aspergillus terreus | octan-2-one + H2O2 | - |
? | |
(R)-2-octanol + O2 | - |
Aspergillus terreus MTCC 6324 | octan-2-one + H2O2 | - |
? | |
3-phenylpropan-1-ol + O2 | - |
Aspergillus terreus | 3-phenylpropanal + H2O2 | - |
? | |
3-phenylpropan-1-ol + O2 | - |
Aspergillus terreus MTCC 6324 | 3-phenylpropanal + H2O2 | - |
? | |
additional information | substrate specificity of native, intermediate dissociated protein and re-associated protein, overview | Aspergillus terreus | ? | - |
? | |
additional information | substrate specificity of native, intermediate dissociated protein and re-associated protein, overview | Aspergillus terreus MTCC 6324 | ? | - |
? | |
n-dodecanol + O2 | - |
Aspergillus terreus | n-dodecanal + H2O2 | - |
? | |
n-dodecanol + O2 | - |
Aspergillus terreus MTCC 6324 | n-dodecanal + H2O2 | - |
? | |
n-heptanol + O2 | - |
Aspergillus terreus | n-heptanal + H2O2 | - |
? | |
n-heptanol + O2 | - |
Aspergillus terreus MTCC 6324 | n-heptanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | the enzyme is composed of several subunits of of 85, 63, 43, 27 and 13 kDa, the 27 kDa subunit is degraded to 25 kDa, overview | Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | essential | Aspergillus terreus |