Any feedback?
Literature summary for 1.1.3.13 extracted from
- Purification and characterization of alcohol oxidase from Paecilomyces variotii isolated as a formaldehyde-resistant fungus (2008), Appl. Microbiol. Biotechnol., 77, 995-1002.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 1.25fold activation | Paecilomyces variotii |  |
| iodoacetamide | 1 mM, 1.3fold activation | Paecilomyces variotii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 0.1 mM, 31% inhibition | Paecilomyces variotii | |
| HgCl2 | 1 mM, 44% inhibition | Paecilomyces variotii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
methanol | - |
Paecilomyces variotii | |
| 3.8 | - |
ethanol | - |
Paecilomyces variotii | |
| 4.9 | - |
formaldehyde | - |
Paecilomyces variotii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
6 * 73000, SDS-PAGE | Paecilomyces variotii |
| 450000 | - |
gel filtration | Paecilomyces variotii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces variotii | - |
isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde | - |
| Paecilomyces variotii RI017 | - |
isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paecilomyces variotii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.42 | - |
- |
Paecilomyces variotii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + O2 | 106% of the activity with methanol | Paecilomyces variotii | acetaldehyde + H2O2 | - |
? | |
| ethanol + O2 | 106% of the activity with methanol | Paecilomyces variotii RI017 | acetaldehyde + H2O2 | - |
? | |
| formaldehyde + O2 | 28% of the activity with methanol | Paecilomyces variotii | formic acid + H2O2 | - |
? | |
| formaldehyde + O2 | 28% of the activity with methanol | Paecilomyces variotii RI017 | formic acid + H2O2 | - |
? | |
| methanol + O2 | - |
Paecilomyces variotii | formaldehyde + H2O2 | - |
? | |
| methanol + O2 | - |
Paecilomyces variotii RI017 | formaldehyde + H2O2 | - |
? | |
| n-propanol + O2 | 33% of the activity with methanol | Paecilomyces variotii | n-propanal + H2O2 | - |
? | |
| n-propanol + O2 | 33% of the activity with methanol | Paecilomyces variotii RI017 | n-propanal + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 73000, SDS-PAGE | Paecilomyces variotii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, enzyme retains full activity | Paecilomyces variotii |
| 60 | - |
30 min, enzyme is almost completely inactivated | Paecilomyces variotii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Paecilomyces variotii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | stable | Paecilomyces variotii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | noncovalently bound flavin different from FAD, FMN, and riboflavin | Paecilomyces variotii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
