Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | competitive, reversible | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
methanol | pH 7.5, 30°C | Ogataea angusta | |
6.6 | - |
ethanol | pH 7.5, 30°C | Ogataea angusta | |
10.5 | - |
formaldehyde | pH 7.5, 30°C | Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
8 * 80000, SDS-PAGE | Ogataea angusta |
620000 | - |
gel filtration | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + O2 | Ogataea angusta | - |
acetaldehyde + H2O2 | - |
? | |
formaldehyde + O2 | Ogataea angusta | - |
formic acid + H2O2 | - |
? | |
methanol + O2 | Ogataea angusta | - |
formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 7.7fold from overproducing Hansenula polymorpha strain C-105 by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
purified enzyme | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + O2 | - |
Ogataea angusta | acetaldehyde + H2O2 | - |
? | |
formaldehyde + O2 | - |
Ogataea angusta | formic acid + H2O2 | - |
? | |
formaldehyde + O2 | low activity | Ogataea angusta | formic acid + H2O2 | - |
? | |
methanol + O2 | - |
Ogataea angusta | formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure analysis, overview | Ogataea angusta |
octamer | 8 * 80000, SDS-PAGE | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
AOX | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ogataea angusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
formaldehyde | pH 7.5, 30°C | Ogataea angusta | |
58 | - |
ethanol | pH 7.5, 30°C | Ogataea angusta | |
60 | - |
methanol | pH 7.5, 30°C | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Ogataea angusta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ogataea angusta | isoelectric focusing | - |
6.1 |