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Literature summary for 1.1.3.13 extracted from

  • Couderc, R.; Baratti, J.
    Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase (1980), Agric. Biol. Chem., 44, 2279-2289.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
Komagataella pastoris
H2O2
-
Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
O2 methanol concentration 1 mM Komagataella pastoris
0.7
-
O2 methanol concentration 10 mM Komagataella pastoris
1
-
ethanol
-
Komagataella pastoris
1
-
O2 methanol concentration 100 mM Komagataella pastoris
1.4
-
methanol oxygen concentration 0.19 mM Komagataella pastoris
3.1
-
methanol oxygen concentration 0.93 mM Komagataella pastoris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Komagataella pastoris 5737
-
cytoplasm
-
Ogataea angusta 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
8 * 80000, SDS-PAGE Komagataella pastoris
675000
-
analytical ultracentrifugation, gel filtration Komagataella pastoris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Komagataella pastoris key enzyme of methanol metabolism formaldehyde + H2O2
-
?
methanol + O2 Ogataea angusta key enzyme of methanol metabolism formaldehyde + H2O2
-
?
methanol + O2 Komagataella pastoris IFP 206 key enzyme of methanol metabolism formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-
Komagataella pastoris IFP 206
-
-
-
Ogataea angusta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
-
Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + O2
-
Ogataea angusta acetaldehyde + H2O2
-
?
ethanol + O2 82% of the activity with methanol Komagataella pastoris acetaldehyde + H2O2
-
?
ethanol + O2 82% of the activity with methanol Komagataella pastoris IFP 206 acetaldehyde + H2O2
-
?
isobutanol + O2 1.2% of the activity with methanol Komagataella pastoris isobutyraldehyde + H2O2
-
?
methanol + O2
-
Komagataella pastoris formaldehyde + H2O2
-
?
methanol + O2
-
Ogataea angusta formaldehyde + H2O2
-
?
methanol + O2 key enzyme of methanol metabolism Komagataella pastoris formaldehyde + H2O2
-
?
methanol + O2 key enzyme of methanol metabolism Ogataea angusta formaldehyde + H2O2
-
?
methanol + O2
-
Komagataella pastoris IFP 206 formaldehyde + H2O2
-
?
methanol + O2 key enzyme of methanol metabolism Komagataella pastoris IFP 206 formaldehyde + H2O2
-
?
n-butanol + O2
-
Ogataea angusta n-butanal + H2O2
-
?
n-butanol + O2 20% of the activity with methanol Komagataella pastoris n-butanal + H2O2
-
?
n-propanol + O2
-
Ogataea angusta n-propanal + H2O2
-
?
n-propanol + O2 43% of the activity with methanol Komagataella pastoris n-propanal + H2O2
-
?
n-propanol + O2 43% of the activity with methanol Komagataella pastoris IFP 206 n-propanal + H2O2
-
?

Subunits

Subunits Comment Organism
octamer
-
Ogataea angusta
octamer 8 * 80000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Komagataella pastoris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 45 half-maximal activity at 18°C and 45°C Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Komagataella pastoris
8.5
-
-
Ogataea angusta

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.3 about half-maximal activity at pH 6.5 and 8.3 Komagataella pastoris
6.7 9.8 about half-maximal activity at pH 6.7 and 9.8 Ogataea angusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 stable Komagataella pastoris
8 10 stable Ogataea angusta
9
-
and above, inactivation Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol FAD per mol of subunit Komagataella pastoris
FAD 1 mol FAD per mol of subunit Ogataea angusta