Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Komagataella pastoris | |
H2O2 | - |
Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
O2 | methanol concentration 1 mM | Komagataella pastoris | |
0.7 | - |
O2 | methanol concentration 10 mM | Komagataella pastoris | |
1 | - |
ethanol | - |
Komagataella pastoris | |
1 | - |
O2 | methanol concentration 100 mM | Komagataella pastoris | |
1.4 | - |
methanol | oxygen concentration 0.19 mM | Komagataella pastoris | |
3.1 | - |
methanol | oxygen concentration 0.93 mM | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Komagataella pastoris | 5737 | - |
cytoplasm | - |
Ogataea angusta | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
8 * 80000, SDS-PAGE | Komagataella pastoris |
675000 | - |
analytical ultracentrifugation, gel filtration | Komagataella pastoris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Komagataella pastoris | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? | |
methanol + O2 | Ogataea angusta | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? | |
methanol + O2 | Komagataella pastoris IFP 206 | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris IFP 206 | - |
- |
- |
Ogataea angusta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.9 | - |
- |
Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + O2 | - |
Ogataea angusta | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | 82% of the activity with methanol | Komagataella pastoris | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | 82% of the activity with methanol | Komagataella pastoris IFP 206 | acetaldehyde + H2O2 | - |
? | |
isobutanol + O2 | 1.2% of the activity with methanol | Komagataella pastoris | isobutyraldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Komagataella pastoris | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Ogataea angusta | formaldehyde + H2O2 | - |
? | |
methanol + O2 | key enzyme of methanol metabolism | Komagataella pastoris | formaldehyde + H2O2 | - |
? | |
methanol + O2 | key enzyme of methanol metabolism | Ogataea angusta | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Komagataella pastoris IFP 206 | formaldehyde + H2O2 | - |
? | |
methanol + O2 | key enzyme of methanol metabolism | Komagataella pastoris IFP 206 | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | - |
Ogataea angusta | n-butanal + H2O2 | - |
? | |
n-butanol + O2 | 20% of the activity with methanol | Komagataella pastoris | n-butanal + H2O2 | - |
? | |
n-propanol + O2 | - |
Ogataea angusta | n-propanal + H2O2 | - |
? | |
n-propanol + O2 | 43% of the activity with methanol | Komagataella pastoris | n-propanal + H2O2 | - |
? | |
n-propanol + O2 | 43% of the activity with methanol | Komagataella pastoris IFP 206 | n-propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Ogataea angusta |
octamer | 8 * 80000, SDS-PAGE | Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Komagataella pastoris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | 45 | half-maximal activity at 18°C and 45°C | Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Komagataella pastoris |
8.5 | - |
- |
Ogataea angusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.3 | about half-maximal activity at pH 6.5 and 8.3 | Komagataella pastoris |
6.7 | 9.8 | about half-maximal activity at pH 6.7 and 9.8 | Ogataea angusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | stable | Komagataella pastoris |
8 | 10 | stable | Ogataea angusta |
9 | - |
and above, inactivation | Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per mol of subunit | Komagataella pastoris | |
FAD | 1 mol FAD per mol of subunit | Ogataea angusta |